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AssigneesBoyce Thompson Institute for Plant Research, Inc., University of Iowa Research Foundation
InventorsPaul McCray, Patrick Sinn, Gary Blissard
Estimating the term…
A method of co-expressing a portion of the VSV G protein gene or a truncated “stem” portion with GP64 and a retrovirus increases the titer of retroviral vectors. A truncated VSV G protein, preferably comprised of a small segment from the C-terminal portion of the ectodomain plus the transmembrane (TM) and cytoplasmic tail (CTD) domains of VSV G, co-expressed with retroviral vectors, enhances the production titers of the retroviral vectors. A preferred embodiment uses a VSV G construct that includes an N-terminal c-Myc epitope plus 42 amino acids from the C-terminal portion of the ectodomain, 20 amino acids from the predicted TM domain, and 29 amino acids from the predicted CTD of the VSV G protein.
A method of producing a plurality of viral particles of a retrovirus, comprising the step of delivering a retroviral packaging plasmid, a transgene plasmid, an envelope protein stem construct and an envelope plasmid into a system capable of producing viral particles, wherein the envelope protein stem construct comprises a Rhabdovirus glycoprotein stem construct and wherein the method results in an increased titer compared to delivering the retroviral packaging plasmid, the transgene plasmid, and the envelope plasmid without the envelope protein stem construct.
A method of producing a plurality of viral particles of a retrovirus, comprising the step of delivering a retroviral packaging plasmid, a transgene plasmid, a G-stem construct and an GP64 envelope plasmid into a system capable of producing viral particles, wherein the G-stem construct comprises a Rhabdovirus glycoprotein stem construct and wherein the method results in an increased titer compared to delivering the retroviral packaging plasmid, the transgene plasmid and the GP64 envelope plasmid without the G-stem construct.
A method of producing a plurality of viral particles of a retrovirus, comprising the step of delivering a plurality of components comprising a retroviral packaging plasmid, a transgene plasmid, an envelope protein stem construct and an envelope plasmid by co-transfecting plasmids, generating stable cell lines, or using other methods to express or otherwise deliver the appropriate proteins, DNA or RNA to produce the viral particles, wherein the envelope protein stem construct comprises a Rhabdovirus glycoprotein stem construct and wherein the method results in an increased titer compared to delivering the retroviral packaging plasmid, the transgene plasmid, and the envelope plasmid without the envelope protein stem construct.
The method of claim , wherein the Vesicular Stomatitis Virus G-stem construct comprises amino acids 421 to 511 of a Vesicular Stomatitis Virus G protein.
Dependencies are inferred from the claim text.