VLPs derived from cells that do not express a viral matrix or core protein
AssigneeNovavax, Inc.
InventorsGale Smith, Peter Pushko, Kutub Mahmood, Bin Zhao
Life of this patent
Estimating the term…
Abstract
The present invention discloses novel influenza virus-like particles (VLPs) that contain chimeric proteins or influenza membrane proteins. The chimeric proteins are derived from fragments of influenza membrane proteins fused to heterologous proteins. The invention includes antigenic formulations and vaccines comprising VLPs of the invention as well as methods of making and administering VLPs to vertebrates, including methods of inducing immunity to infections, such as influenza.
Claims20
A method of making a virus-like particle (VLP) in a cell that comprises at least two influenza membrane proteins, comprising:
- expressing at least a first influenza membrane protein and a second influenza membrane protein in a cell, wherein said first influenza membrane protein is hemagglutinin (HA) and said second influenza membrane protein is neuraminidase (NA) to obtain assembled VLPs;
- and
- purifying the assembled VLPs, wherein the cell does not express any viral matrix or core protein.
Dependencies are inferred from the claim text.