Flavivirus reporter virus and methods of making and using the same
AssigneeIntegral Molecular, Inc.
InventorsBridget Puffer, Kimberly-Anne Mattia
Life of this patent
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Abstract
The present invention relates to the production and uses of flavivirus replicons and flavivirus particles and reporter virus particles. The present invention relates to the production and uses of chimeric and codon-optimized flavivirus virus replicons and flavivirus virus particles and reporter virus particles.
Claims16
An isolated nucleic acid molecule comprising a nucleic acid molecule that comprises a sequence that is at least 95% identical to a sequence comprising SEQ ID NO:
- 1 or SEQ ID NO:
- 3.
A method of producing a dengue reporter virus particle comprising transfecting a producer cell with a plasmid DNA molecule comprising a nucleic acid molecule comprising SEQ ID NO:
- 1 or 3 and optionally isolating the reporter virus particles in the supernatant from said transfected producer cell,
- wherein said producer cell takes up the plasmid DNA molecule, expresses a replicon of DEN, and produces DENV RVPs that are released from said producer cell.
Dependencies are inferred from the claim text.