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AssigneeIkeda Food Research Co., Ltd.
InventorsHironori Omura, Hirokazu Sanada, Takako Yada, Ayaka Atsumi, Tetsunari Morita, Emi Ishimaru
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The present invention provides members that produce on a large scale a coenzyme-linked glucose dehydrogenase which has excellent substrate-recognizing ability toward glucose while providing low action on maltose. The present invention relates to a polynucleotide encoding a soluble coenzyme-linked glucose dehydrogenase that catalyzes the oxidation of glucose in the presence of an electron acceptor and has an activity toward maltose of 5% or lower; a polypeptide encoded by the nucleotide sequence of the polynucleotide; a recombinant vector carrying the polynucleotide; a transformed cell produced using the recombinant vector; a method for producing a polypeptide comprising culturing the transformed cell and collecting from the cultivated products a polypeptide that links to FAD to exert the glucose dehydration activity; a method for determination of glucose using the polypeptide; a reagent composition for determination of glucose; and a biosensor.
A cDNA encoding a coenzyme-linked glucose dehydrogenase, comprising the sequences set forth in SEQ ID NOs. 5 to 7, the coenzyme-linked glucose dehydrogenase having the following properties (a) to (d):
A cDNA encoding a flavin adenine dinucleotide (FAD)-linked glucose dehydrogenase (GLD), the GLD comprising sequences set forth in SEQ ID NOs. 8 to 12, the coenzyme-linked glucose dehydrogenase having following properties (a) to (c):
A cDNA encoding a flavin adenine dinucleotide (FAD)-linked glucose dehydrogenase (GLD) which catalyzes dehydrogenation of glucose in presence of an electron acceptor and having an activity toward maltose of 5% or less with respect to an activity toward glucose,
A cDNA encoding a flavin adenine dinucleotide (FAD)-linked glucose dehydrogenase (GLD) which catalyzes dehydrogenation of glucose in presence of an electron acceptor and having an activity toward maltose of 5% or less with respect to an activity toward glucose, the cDNA comprising the nucleotide sequence set forth in SEQ ID NO. 1.
A cDNA encoding a flavin adenine dinucleotide (FAD)-linked glucose dehydrogenase (GLD) which catalyzes dehydrogenation of glucose in presence of an electron acceptor and having an activity toward maltose of 5% or less with respect to an activity toward glucose,
A cDNA encoding a flavin adenine dinucleotide (FAD)-linked glucose dehydrogenase (GLD) catalyzing dehydrogenation of glucose in presence of an electron acceptor and having an activity toward maltose of 5% or less with respect to an activity toward glucose,
Dependencies are inferred from the claim text.