Tissue engineering methods and compositions
AssigneeCytex Therapeutics, Inc.
InventorsFarshid Guilak, Bradley T. Estes, Franklin Thomas Moutos
Life of this patent
Estimating the term…
Abstract
The presently disclosed subject matter generally relates to methods and systems for facilitating the growth and differentiation of adipose-derived stem cells for laboratory and therapeutic applications. The cells can be employed alone or in conjunction with unique biologically-compatible scaffold structures to generate differentiated tissues and structures, both in vitro and in vivo. The presently disclosed subject matter further relates to methods of forming and using improved tissue engineered scaffolds that can be used as substrates to facilitate the growth and differentiation of cells.
Claims25
A method of resurfacing a predetermined articular joint in a subject, the joint having a number of focal defects, the method comprising:
- (a) providing an implant comprising:
- (i) a scaffold comprising a woven fibrous biocompatible material sized to replace substantially all of the articulating surface of the predetermined joint, and shaped substantially like said articulating surface of said predetermined joint;
- and
- (ii) one or more adipocyte stem cells ;
- (b) infiltrating the one or more of said cells throughout the scaffold;
- and
- (c) implanting at a site of the joint in the subject the implant provided in steps (a) and (b) to thereby resurface said articulating surface of the joint in the subject encompassing the number of focal defects.
A method of resurfacing a predetermined articular joint in a subject, the joint having a number of focal defects, the method comprising:
- (a) providing an implant comprising:
- (i) a scaffold comprising a woven fibrous biocompatible material and sized to replace substantially all of the articulating surface of the predetermined joint and shaped substantially like said articulating surface of said predetermined joint;
- and
- (ii) one or more adipocyte stem cells;
- (b) encouraging infiltration of the one or more cells throughout the scaffold;
- (c) devitalizing a fraction of the cells before implantation;
- and
- (d) implanting at a site of the joint in the subject the implant provided in steps (a), (b), and (c) to thereby resurface said articulating surface of the joint in the subject encompassing the number of focal defects.
Dependencies are inferred from the claim text.