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US 8,685,726 B2Granted
Small molecules supporting pluripotent cell growth and methods thereof
AssigneeViaCyte, Inc.
InventorsThomas C Schulz, Allan J Robins
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Abstract
The present invention relates to compositions and methods for maintaining undifferentiated pluripotent stem cell cultures.
Claims7
A composition comprising:
- (a) a defined cell culture medium that is sufficient to achieve undifferentiated expansion of human pluripotent cells cultured therein;
- (b) one or more human pluripotent cells present at low cell density in the defined cell culture medium;
- and
- (c) norepinephrine or a structural analog, derivative, or physiologically acceptable salt thereof present in the defined culture medium in an amount that is sufficient to achieve low density survival and expansion of pluripotent cells.
1 more element
A method for expanding a human pluripotent cell, the method comprising the step of incubating one or more human pluripotent cells present at low cell density in a composition comprising:
- (a) a defined cell culture medium that is sufficient to achieve undifferentiated expansion of human pluripotent cells cultured therein;
- and
- (b) norepinephrine or a structural analog, derivative, or physiologically acceptable salt thereof present in the defined culture medium in an amount that is sufficient to achieve low density survival and expansion of pluripotent cells.
Dependencies are inferred from the claim text.