Methods and compositions for in vitro and in vivo chondrogenesis
AssigneeBioTime, Inc.
InventorsMichael D. West, Hal Sternberg, Karen B. Chapman
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Abstract
Aspects of the present invention include methods and compositions related to the production, identification and use of embryonic progenitor cell lines that are capable of undergoing chondrogenesis. A number of exemplary chondrogenic cell lines derived from primordial stem cells are disclosed. The chondrogenic cell lines described herein are robust, can expand for >40 passages, and have site-specific purity, thus providing for compositions and methods of producing diverse cartilage types with unique molecular compositions for use in research and therapy.
Claims6
A method of producing cartilage, the method comprising culturing a clonally-purified embryonic progenitor cell line;
- wherein said cell line is negative for the expression of one or more of the genes selected from the group consisting of:
- CD74, CD90, CD166, ITGA2, and KCNK2;
- wherein said cell line is selected from the group consisting of:
- SM30, E15, 4D20.8, 7SM0032, MEL2, SK11 and combinations thereof;
- and wherein said culturing comprises culture under cartilage producing conditions for an amount of time effective to induce cartilage production from said cells.
Dependencies are inferred from the claim text.