Lanthanide mass dots: nanoparticle isotope tags
AssigneeThe Board of Trustees of the Leland Stanford Junior University
InventorsGarry P. Nolan, Erin F. Simonds, Sean C. Bendall
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Abstract
Compositions and methods are provided for the use of nanoparticles, which may be referred to herein as mass dots, as mass tags for probes such as antibodies, aptamers, nucleic acids, etc. in multiplexed bioassays with ICP-MS detection.
Claims16
A probe comprising a specific binding member for an analyte, conjugated at a single linkage site to a mass dot, wherein the mass dot is a solid lanthanide metal particle of at least 100 metal atoms, free of high atomic mass dopants, and having an isotopic purity between about 90 and 100%.
A probe comprising a specific binding member for an analyte, conjugated at a single linkage site to a mass dot, wherein the mass dot is a solid lanthanide particle of at least 100 metal atoms, free of high atomic mass dopants, wherein said mass dot is comprised of non-fluorescent nanocrystal of two or more lanthanide metals at a known ratio and absolute quantity.
A probe comprising a specific binding member for an analyte, conjugated at a single linkage site to a mass dot, wherein the mass dot is a solid lanthanide particle of at least 100 metal atoms, free of high atomic mass dopants, wherein said mass dot comprises a nanoparticle core of said lanthanide metal, and a low molecular weight counter-anion of a molecular weight outside the detection range of ICP-MS.
A method for the detection of an analyte, the method comprising:
- binding the analyte through specific binding to 10 or more distinct probes, wherein each probe comprises a specific binding member conjugated at a single linkage site to at least one mass dot and not more than 10 mass dots, wherein the mass dot is a solid lanthanide particle of at least 100 metal atoms, free of high atomic mass dopants, and having an isotopic purity between about 90 and 100%,
- wherein said detecting is performed by ICP-MS with solution analysis or by mass cytometry.
Dependencies are inferred from the claim text.