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US 8,673,557 B2Granted
Coupling endonucleases with end-processing enzymes drives high efficiency gene disruption
AssigneeSeattle Children's Research Institute
InventorsAndrew M. Scharenberg, Michael T. Certo, Kamila S. Gwiazda
Life of this patent
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Abstract
The present disclosure relates to the co-expression of an endonuclease with an end-processing enzyme for the purpose of enhanced processing of the polynucleotide ends generated by endonuclease cleavage.
Claims14
A method of increasing mutagenesis of a nucleotide sequence at a selected nucleic acid site in a cell comprising:
- selecting a sequence for targeted mutagenesis in said cell;
- selecting an endonuclease that cleaves at said selected nucleic acid site;
- and coupling the activity of said endonuclease and the activity of one or more end-processing enzymes within said cell so as to increase mutagenesis at said selected nucleic acid site.
Dependencies are inferred from the claim text.