Back to results
US 8,673,315 B2Granted
Method for producing vaccinal viral strain of a virus of the reoviridae family
AssigneeLondon School of Hygiene and Tropical Medicine
InventorsPolly Roy, Mark Boyce
Life of this patent
Estimating the term…
Abstract
The invention relates to a method for producing a modified viral strain of a virus which is a member of the Reoviridae family and, in particular, relates to vaccinal viral strains of the Orbivirus genus.
Claims14
A method for producing a vaccinal viral strain of a virus which is a member of the Reoviridae family, the method comprising:
- introducing a mutation into the Reoviridae virus so that the function of an essential gene is destroyed;
- transfecting a cell with viral single stranded RNA (ss RNA) derived from the virus genome and comprising the mutation, wherein the transfection does not use a helper virus, and wherein transfecting the cell comprises two or more transfection steps, wherein:
- (1) a first transfection step comprises transfecting the cell with ss RNA encoding components required for assembly of an inner viral capsid layer;
- and
- (2) a second transfection step comprises transfecting the cell with ss RNA which is a transcript of the viral genome and comprises the mutation, and encodes all the components required for assembly of the virus;
- and
- culturing the transfected cell under suitable conditions in order to lead to the production of the vaccinal viral strain,
- wherein the cell complements the function of the essential viral gene thereby allowing the vaccinal viral strain to replicate in the cell.
5 more elements
A method for producing a modified viral strain of a virus which is a member of the Reoviridae family, the method comprising:
- introducing a modification into the Reoviridae virus;
- transfecting a cell with viral single stranded RNA (ss RNA) derived from the virus genome and comprising the modification, wherein the transfection does not use a helper virus, and wherein transfecting the cell comprises two or more transfection steps, wherein:
- (1) a first transfection step comprises transfecting the cell with ss RNA encoding components required for assembly of an inner viral capsid layer;
- and
- (2) a second transfection step comprises transfecting the cell with ss RNA which is a transcript of the viral genome and comprises the modification, and encodes all the components required for assembly of the virus;
- and
- culturing the transfected cell under suitable conditions in order to lead to the production of the modified viral strain,
- wherein the cell allows the modified viral strain to replicate in the cell.
5 more elements
Dependencies are inferred from the claim text.