Oplophorus-derived luciferases, novel coelenterazine substrates, and methods of use
AssigneePromega Corporation
InventorsBrock Binkowski, Lance P. Encell, Mary Hall, Matthew B. Robers, Michael R. Slater, Keith V. Wood, Monika G. Wood
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Abstract
An isolated polynucleotide encoding a modified luciferase polypeptide and substrates. The OgLuc variant polypeptide has at least 60% amino acid sequence identity to SEQ ID NO: 1 and at least one amino acid substitution at a position corresponding to an amino acid in SEQ ID NO: 1. The OgLuc variant polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the corresponding polypeptide of the wild-type Oplophorus luciferase.
Claims22
An isolated polynucleotide encoding a polypeptide having at least 80% sequence identity to SEQ ID NO:
- 1 comprising one or more amino acid substitutions relative to SEQ ID NO:
- 3, wherein at least one amino acid substitution is at a position corresponding to position 18 of SEQ ID NO:
- 1, wherein the polypeptide has enhanced luminescence relative to SEQ ID NO:
- 3.
Dependencies are inferred from the claim text.