# US 8,658,411 B2 · Method of treating wastewater containing organic compound > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 8,658,411 B2 - Document ID: US8658411B2 - Status: Granted - Kind: B2 - Application number: 11/721,631 - Filed: 2006-04-21 - Published: 2010-02-25 - Granted: 2014-02-25 - Earliest claimed: 2005-04-21 - Assignees: Ibiden Co., Ltd. - Applicants: Ryozo Irie; Michio Tabata; Yoshihisa Hibi - Inventors: Ryozo Irie; Michio Tabata; Yoshihisa Hibi - Web page: https://patentgrep.com/patent/US8658411B2 ## CPC - C12N 1/24: Processes using, or culture media containing, waste sulfite liquor - C02F 3/1273: {Submerged membrane bioreactors} - C02F 3/34: characterised by the microorganisms used - C02F 2101/32: Hydrocarbons, e.g. oil - C02F 2101/38: containing nitrogen - C02F 2303/02: Odour removal or prevention of malodour - C02F 2305/06: Nutrients for stimulating the growth of microorganisms - Y02W 10/10: Biological treatment of water, waste water, or sewage - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) - C02F: TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE (separation in general B01D; special arrangements on waterborne vessels of installations for treating water, waste water or sewage, e.g. for producing fresh water, B63J; adding materials to water to prevent corrosion C23F; treating radioactively-contaminated liquids G21F9/04; regeneration of reactants for recirculation into processes, see the relevant places for the processes) - Y02W: CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT ## Abstract The problem addressed by the present invention is to provide a method of treating an organic substance-containing waste liquid, and a treatment apparatus therefor, and an additive for treating an organic substance-containing waste liquid, and a bacterium for degrading an organic substance component or the like in the waste liquid, capable of accomplishing at least one of treatment of the organic substance-containing waste liquid with a high efficiency and a high treatment rate, without substantially generating a foul odor, and obtainment of water quality that is suitable for releasing to sewage, a public water area, or the like. This problem is solved by providing a method of treating an organic substance-containing waste liquid characterized by contacting an activated sludge containing a specified bacterium with an organic substance-containing waste liquid. The invention also provides a treatment apparatus therefor and an additive for treating an organic substance-containing waste liquid which is used by adding the additive to an activated sludge in a treatment tank in which a specified bacterium is present, and a specified bacterium for degrading an organic substance-containing waste liquid. ## Claims ### Claim 1 A method of treating an organic substance containing waste liquid, comprising: contacting an organic substance containing waste liquid and an activated sludge to produce a mixture, wherein said activated sludge comprises bacteria from (i) an Alcaligenes selected from the group consisting of Alcaligenes faecalis, Alcaligenes pacifica, Alcaligenes aquamarina and Alcaligenes venustus, (ii) a Sphingobacterium selected from the group consisting of Sphingobacterium faecium, Sphingobacterium spiritivorum, Sphingobacterium multivorum, and Sphingobacterium mizutae, (iii) Shewanella algae, (iv) a Rhodobacter selected from the group consisting of Rhodobacter litoralis, Rhodobacter veldkampii, Rhodobacter massiliensis, Rhodobacter sphaeroides, and Rhodobacter azotoformans, (v) Micrococcus luteus, (vi) a Paracoccus selected from the group consisting of Paracoccus denitrificans, Paracoccus pantotrophus, and Paracoccus thiophilus, (vii) Bosea thiooxidans, and (viii) Paracoccus verustus, wherein the Alcaligenes is bacterial strain IBI-2P, Accession No. FERM BP-10565, the Sphingobacterium is bacterial strain IBI-3P, Accession No. FERM BP-10566, the Shewanella algae is bacterial strain IBI-6P, Accession No. FERM BP-10568, the Rhodobacter is bacterial strain IBI-15P, Accession No. FERM BP-10570, the Micrococcus luteus is bacterial strain IBI-40P, Accession No. FERM BP-10571, the Paracoccu is bacterial strain IBI-6, Accession No. FERM BP-10567, the Bosea thiooxidans is bacterial strain IBI-13 designated as Accession No. FERM BP-10569, and the Paracoccus verustus is bacterial strain IBI-2, Accession No. FERM BP-10564. ### Claim 2 (depends on 1) The method of claim 1, wherein the organic substance containing waste liquid is a waste liquid containing at least one compound selected from the group consisting of higher alcohols, sulfide compounds, alkylthiosulfate compounds, nitrogen containing alkyl compounds, phosphoric acid compounds, diethylene glycol monobutyl ether, dipropylene glycol ether, monoethanolamine, polyoctyl phenyl ethers having an average molecular weight of from 696 to 872, isopropyl alcohol, ethylene glycol, N,N′-dimethylformamide, and benzimidazoles having an alkyl group having 1 to 4 carbon atoms. ### Claim 3 (depends on 1) The method of claim 1, wherein the organic substance containing waste liquid is raw sewage, domestic waste water, waste water from a food-processing factory, or a degreasing agent containing waste liquid. ### Claim 4 (depends on 1) The method of claim 1, wherein said mixture has dissolved oxygen (DO) content of from 2 to 8 mg/L. ### Claim 5 (depends on 1) The method of claim 1, further comprising adding a magnesium compound, a silicon containing compound and a nutritional supplement for cell culture to said mixture. ### Claim 6 (depends on 1) The method of claim 1, wherein the organic substance containing waste liquid has an original waste water with a pH of from 3.5 to 8.5. ### Claim 7 (depends on 1) The method of claim 1, wherein said mixture has a pH of from 6.0 to 9.0 or is adjusted to have a pH from 6.0 to 9.0. ### Claim 8 (depends on 1) The method of claim 1, wherein said mixture has an oxidation-reduction potential (ORP) inside a treatment tank of from −100 to 150 mv or is adjusted to an ORP of from −100 mv to 150 mv. ### Claim 9 (depends on 1) The method of claim 1, wherein said mixture has a temperature of from 10° to 60° C. ### Claim 10 (depends on 1, 2, 3, 4, 5, 6, 7, 8, 9) The method of any one of claims 1 to 9, wherein the organic substance containing waste liquid is previously subjected to a step of removing a metal. ### Claim 11 (depends on 1, 2, 3, 4, 5, 6, 7, 8, 9) The method of any one of claims 1 to 9, wherein a resulting waste liquid from said mixture is then filtered with a submerged membrane. ### Claim 12 (depends on 1, 2, 3, 4, 5, 6, 7, 8, 9) An apparatus for treating an organic substance containing waste liquid in accordance with the method of any one of claims 1 to 9 comprising: (a) a treatment tank containing an activated sludge comprising the bacteria (i) an Alcaligenes selected from the group consisting of Alcaligenes faecalis, Alcaligenes pacifica, Alcaligenes aquamarina and Alcaligenes venustus, (ii) a Sphingobacterium selected from the group consisting of Sphingobacterium faecium, Sphingobacterium spiritivorum, Sphingobacterium multivorum, and Sphingobacterium mizutae, (iii) Shewanella algae, (iv) a Rhodobacter selected from the group consisting of Rhodobacter litoralis, Rhodobacter veldkampii, Rhodobacter massiliensis, Rhodobacter sphaeroides, and Rhodobacter azotoformans, (v) Micrococcus luteus, (vi) a Paracoccus selected from the group consisting of Paracoccus denitrificans, Paracoccus pantotrophus, and Paracoccus thiophilus, (vii) Bosea thiooxidans, and (viii) Paracoccus verustus; and (b) a means of filtering a waste liquid in the treatment tank with a submerged-membrane. ### Claim 13 (depends on 10) An apparatus for treating an organic substance-containing waste liquid in accordance with claim 10 which comprises (a) a treatment tank containing an activated sludge comprising bacteria of (i) an Alcaligenes species selected from the group consisting of Alcaligenes faecalis, Alcaligenes pacifica, Alcaligenes aquamarina and Alcaligenes venustus, (ii) a Sphingobacterium species selected from the group consisting of Sphingobacterium faecium, Sphingobacterium spiritivorum, Sphingobacterium multivorum, and Sphingobacterium mizutae, (iii) Shewanella algae, (iv) a Rhodobacter species selected from the group consisting of Rhodobacter litoralis, Rhodobacter veldkampii, Rhodobacter massiliensis, Rhodobacter sphaeroides, and Rhodobacter azotoformans, (v) Micrococcus luteus, (vi) a Paracoccus species selected from the group consisting of Paracoccus denitrificans, Paracoccus pantotrophus, and Paracoccus thiophilus, (vii) Bosea thiooxidans, and (viii) Paracoccus verustus; and (b) a means of filtering a waste liquid in the treatment tank with a submerged membrane. ### Claim 14 (depends on 11) An apparatus for treating an organic substance containing waste liquid in accordance with claim 11 which comprises (a) a treatment tank containing an activated sludge comprising bacteria of (i) an Alcaligenes species selected from the group consisting of Alcaligenes faecalis, Alcaligenes pacifica, Alcaligenes aquamarina and Alcaligenes venustus, (ii) a Sphingobacterium species selected from the group consisting of Sphingobacterium faecium, Sphingobacterium spiritivorum, Sphingobacterium multivorum, and Sphingobacterium mizutae, (iii) Shewanella algae, (iv) a Rhodobacter species selected from the group consisting of Rhodobacter litoralis, Rhodobacter veldkampii, Rhodobacter massiliensis, Rhodobacter sphaeroides, and Rhodobacter azotoformans, (v) Micrococcus luteus, (vi) a Paracoccus species selected from the group consisting of Paracoccus denitrificans, Paracoccus pantotrophus, and Paracoccus thiophilus, (vii) Bosea thiooxidans, and (viii) Paracoccus verustus; and (b) a means of filtering a waste liquid in the treatment tank with a submerged membrane.