Methods and compositions for producing fatty alcohols and fatty aldehydes
AssigneeLS9, Inc.
InventorsAndreas W. Schirmer, Mathew A. Rude, Shane A. Brubaker
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Abstract
Compositions and methods for producing hydrocarbons such as aldehydes, alkanes, and alkenes are described herein. Certain hydrocarbons can be used in biofuels.
Claims26
A method of producing a fatty aldehyde or a fatty alcohol, the method comprising:
- (a) expressing a gene encoding a polypeptide that catalyzes the conversion of an acyl-ACP to a fatty aldehyde in an engineered microorganism;
- (b) culturing said engineered microorganism in a culture media containing a carbohydrate carbon source under conditions effective to produce a fatty aldehyde or a fatty alcohol in the culture media, wherein said polypeptide comprises an amino acid sequence having at least 90% sequence identity to SEQ ID NO:
- 66.
A cell culture comprising a recombinant microorganism and a culture medium containing a carbon source, wherein a polypeptide that catalyzes the conversion of an acyl-ACP to a fatty aldehyde is overexpressed in said recombinant microorganism and a fatty aldehyde or a fatty alcohol is produced in the cell culture when said recombinant microorganism is cultured in the culture medium under conditions effective to express said polypeptide, wherein said polypeptide comprises an amino acid sequence having at least 90% sequence identity to SEQ ID NO:
- 66.
A cell culture comprising a recombinant microorganism and culture medium containing a carbon source,
- wherein a polypeptide that catalyzes the conversion of an acyl-ACP to a fatty aldehyde is overexpressed in said recombinant microorganism and a fatty aldehyde or a fatty alcohol is produced in the cell culture medium when said recombinant microorganism is cultured under conditions effective to express said polypeptide and wherein said polypeptide comprises amino acids 126-136 and 138-143 of SEQ ID NO:
- 66.
A cell culture comprising a recombinant microorganism and culture medium containing a carbon source, wherein a polypeptide that catalyzes the conversion of an acyl-ACP to a fatty aldehyde is overexpressed in said recombinant microorganism and a fatty aldehyde or a fatty alcohol is produced in the cell culture medium when said recombinant microorganism is cultured under conditions effective to express said polypeptide and wherein said polypeptide comprises amino acids 156-159 and 161-175 of SEQ ID NO:
- 66.
A cell culture comprising a recombinant microorganism and culture medium containing a carbon source, wherein a polypeptide that catalyzes the conversion of an acyl-ACP to a fatty aldehyde is overexpressed in said recombinant microorganism and a fatty aldehyde or a fatty alcohol is produced in the cell culture medium when said recombinant microorganism is cultured under conditions effective to express said polypeptide and wherein said polypeptide comprises amino acids 156-175 of SEQ ID NO:
- 66.
Dependencies are inferred from the claim text.