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US 8,652,816 B2Granted
Compositions and methods for 3-hydroxypropionate bio-production from biomass
AssigneeOPX Biotechnologies, Inc.
InventorMichael D. Lynch
Life of this patent
Estimating the term…
Abstract
Methods of obtaining mutant nucleic acid sequences that demonstrate elevated oxaloacetate α-decarboxylase activity are provided. Compositions, such as genetically modified microorganisms that comprise such mutant nucleic acid sequences, are described, as are methods to obtain the same.
Claims15
A method for producing an acrylic acid-based consumer product, said method comprising the steps of:
- i) combining a carbon source and a cell culture to produce 3-hydroxypropionic acid, wherein said cell culture comprises a genetically modified Escherichia coli (E. coli) microorganism comprising a heterologous nucleic acid encoding for a protein with malonyl-CoA reductase activity;
- and wherein said genetically modified E. coli microorganism further comprises reduction in activity of pyruvate formate-lyase;
- ii) growing said cell culture in feed-batch, batch mode, or continuous batch mode production;
- iii) converting said 3-hydroxypropionic acid to acrylic acid;
- iv) polymerizing acrylic acid into a polymer of acrylate;
- and
- v) processing said polymer of acrylate into a consumer product.
4 more elements
A consumer product produced according to the following steps:
- i) combining a carbon source and a cell culture to produce 3-hydroxypropionic acid, wherein said cell culture comprises a genetically modified Escherichia coil microorganism comprising a heterologous nucleic acid encoding for a protein with malonyl-CoA reductase activity;
- and wherein said genetically modified E. coli microorganism further comprises reduction in activity of pyruvate formate-lyase;
- ii) growing said cell culture in feed-batch, batch mode, or continuous batch mode production;
- iii) converting said 3-hydroxypropionic acid to acrylic acid;
- iv) polymerizing acrylic acid into a polymer of acrylate;and
- v) processing said polymer of acrylate into a consumer product.
3 more elements
Dependencies are inferred from the claim text.