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US 8,652,500 B2Granted
Particulate tissue graft with components of differing density and methods of making and using the same
AssigneeACell, Inc.
InventorsRodney W. Bosley, Jr., Clay Fette, Robert S. Tullius
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Abstract
Disclosed are tissue graft compositions made of particles having different densities, methods of making these compositions, and methods of using these compositions for promoting tissue restoration in a patient.
Claims10
A method for preparing a mixture of bioresorbable particulate matter, comprising:
- (a) providing a first sheet of bioresorbable material comprising a native extracellular matrix comprising epithelial basement membrane and tunica propria and having a first density and a first rate of resorption, and,
- milling said first sheet into a first plurality of bioresorbable particles;
- (b) providing a second sheet of a bioresorbable material comprising another extracellular matrix different than the extracellular matrix material of the first sheet and having a second density and a second rate of resorption, and,
- milling said second sheet into a second plurality of bioresorbable particles;
- (c) providing a predetermined quantity of said first plurality of bioresorbable particles and a predetermined quantity of said second plurality of bioresorbable particles;
- and
- (d) mixing said predetermined quantity of said first plurality of bioresorbable particles with a predetermined quantity of said second plurality of bioresorbable particles,
- wherein the second density is 150%-500% of the first density.
5 more elements
A method for preparing a mixture of bioresorbable particulate matter having differing densities comprising:
- providing a first sheet of bioresorbable material comprising a native extracellular matrix comprising epithelial basement membrane and tunica propria and having a first density, and,
- compressing a portion of said sheet such that said portion comprises a second density;
- providing a second sheet of bioresorbable material comprising a native extracellular matrix;
- joining said first sheet to said second sheet to form a multilaminate sheet, and
- milling said multilaminate sheet into a plurality of bioresorbable particles, wherein said mixture of bioresorbable particles promotes tissue restoration and repair when implanted at a tissue site in a mammalian patient, wherein the second density of the first sheet is at least 150% of the first density.
2 more elements
A method of preparing a mixture of bioresorbable extracellular matrix particles, comprising:
- preparing a plurality of bioresorbable particles from a native extracellular matrix comprising epithelial basement membrane and tunica propria each having the same density;
- dividing the plurality of bioresorbable particles into a first group and a second group;
- coating the first group of said plurality of bioresorbable particles with a coating comprising a material selected from the group consisting of an extracellular matrix, polyglycolic acid, hyaluronic acid, collagen, or combinations thereof, said coating having a first density;
- coating the second group of said plurality of bioresorbable particles with a coating comprising a material selected from the group consisting of an extracellular matrix, polyglycolic acid, hyaluronic acid, collagen, or combinations thereof, said coating having a second density;
- mixing said first group of bioresorbable particles with said second group of bioresorbable particles to form a mixture of bioresorbable particles, wherein said mixture of bioresorbable particles promotes tissue restoration and repair when implanted at a tissue site in a mammalian patient, and wherein the second density is 150%-500% of the first density.
2 more elements
Dependencies are inferred from the claim text.