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US 8,647,870 B2Granted
Use of biological photoreceptors as directly light-activated ion channels
AssigneeMax-Planck-Gesellschaft zur Forderung der Wissenschaften e.V.
InventorsPeter Hegemann, Georg Nagel, Ernst Bamberg
Life of this patent
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Abstract
A biological photoreceptor, which is a directly light-controlled ion channel, including (i) an apoprotein and (ii) a light-sensitive polyene covalently bound to the apoprotein, the polyene interacting with the apoprotein and functioning as a direct light-sensitive gate.
Claims19
A biological photoreceptor, which is a directly light-controlled ion channel, comprising (i) an apoprotein that comprises amino acids 61 to 310 of SEQ ID NO:1 that is Channelopsin 1 (CHOP-1) protein;
- and (ii) a light-sensitive polyene covalently bound to the apoprotein, said polyene interacting with the apoprotein and functioning as a direct light-sensitive gate, wherein the light-sensitive polyene is a retinal derivative selected from the group consisting of 3,4-dehydroretinal, 13-ethylretinal, 9-dm-retinal, 3-hydroxyretinal, 4-hydroxyretinal, naphthylretinal;
- 3,7,11-trimethyl-dodeca-2,4,6,8,10-pentaenal;
- 3,7-dimethyl-deca-2,4,6,8-tetraenal;
- 3,7-dimethyl-octa-2,4,6-trienal;
- and 6-7 rotation-blocked retinals, 8-9 rotation-blocked retinals, and 10-11 rotation-blocked retinal.
2 more elements
A biological photoreceptor, which is a directly light-controlled ion channel, comprising:
- (i) an amino acid sequence having at least five transmembrane helices, wherein said amino acid sequence comprises one or more conservative amino acid changes relative to the corresponding region of SEQ ID NO:
- 2 provided that the amino acid sequence has at least 52.7% identity to SEQ ID NO:2, and comprises aspartic acid at position 253 of SEQ ID NO:2, lysine at position 257 of SEQ ID NO:2, tryptophan at position 260 of SEQ ID NO:2, glutamic acid at position 123 of SEQ ID NO:2, and arginine at position 134 of SEQ ID NO:2;
- and
- (ii) a light-sensitive retinal or retinal derivative.
1 more element
Dependencies are inferred from the claim text.