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US 8,647,855 B2Granted
Genetically engineered yeast for the production of biofuels
AssigneeNew England Biolabs, Inc.
InventorsChristopher H. Taron, Paul A. Colussi
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Abstract
Compositions and methods are provided for generating biofuels by fermentation from carbon sources other than glucose using genetically engineered yeast strains. For example, a Saccharomyces strain which is capable of converting glucose to ethanol but not of metabolizing N-acetyl glucosamine is genetically engineered to utilize N-acetyl glucosamine as a nutrient carbon source.
Claims16
A composition, comprising:
- a genetically engineered Saccharomyces cell containing heterologous DNA encoding an N-acetyl glucosamine (GlcNAc) kinase, an N-acetylglucosamine-6-phosphate deacetylase and a glucosamine-6-phosphate deaminase.
A method for making a biofuel, comprising:
- modifying a Saccharomyces yeast cell that naturally does not metabolize GlcNAc by introducing heterologous DNA into the Saccharomyces yeast-cell, the heterologous DNA encoding an N-acetyl glucosamine (GlcNAc) kinase, an N-acetylglucosamine-6-phosphate deacetylase and a glucosamine-6-phosphate deaminase;
- providing GlcNAc as a source of nutrient carbon to the modified Saccharomyces yeast cell;
- and
- permitting metabolic conversion of the GlcNAc to the biofuel by propagation of the modified Saccharomyces yeast cell.
1 more element
Dependencies are inferred from the claim text.