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US 8,647,638 B2Granted
Synergistic attenuation of vesicular stomatitis virus, vectors thereof and immunogenic compositions thereof
AssigneeWyeth
InventorsDavid Kirkwood Clarke, Roger Michael Hendry, Stephen A. Udem, Christopher Lee Parks
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Abstract
The present invention broadly relates to the synergistic attenuation of vesicular stomatitis virus (VSV), More particularly, the invention relates to the identification of combined mutation classes which synergistically attenuate the pathogenicity of VSV vectors in mammals and immunogenic compositions thereof.
Claims8
A genetically modified vesicular stomatitis virus (VSV) vector comprising at least two different classes of mutations in the same VSV genome, wherein the VSV vector comprises:
- (a) a truncated G gene mutation, wherein the G protein encoded by the truncated G gene has a cytoplasmic tail domain consisting of one amino acid and,
- (b) a non-cytopathic M gene (M(ncp)) mutation, wherein the M(ncp) mutation is a mutation at methionine 33 and a mutation at methionine 51 of the M gene, and optionally
- (c) at least one foreign RNA sequence inserted into or replacing a region of the VSV genome non-essential for replication,
- wherein the at least two mutations synergistically attenuate VSV pathogenicity.
1 more element
Dependencies are inferred from the claim text.