OxyVu-1 hyperspectral tissue oxygenation (HTO) measurement system

A no invasive, tissue oxygenation measurement system comprising:

• (A) a hyperspectral/multispectral imaging camera configured to acquire a series of images of a tissue of a subject, the series of images forming a hyperspectral imaging cube;
• (B) one or more processors in electronic communication with the hyperspectral/multispectral imaging camera;
• (C) a memory storing one or more programs for execution by the one or more processors;
• and
• (D) a display configured to display a two-dimensional image of tissue oxygenation of a tissue of a subject imaged by the hyperspectral/multispectral imaging camera, wherein the one or more programs comprise:
• instructions for resolving a plurality of absorption signals from the series of images of the tissue of the subject,
• instructions for correcting background noise from each respective absorption signal in the plurality of absorption signals by subtracting the contribution provided by melanin and the losses provided by diffuse scattering from the respective absorption signal, wherein the contribution provided by melanin and the losses provided by diffuse scattering to the plurality of tissue oxygenation measurements are collectively modeled as a second order polynomial, thereby forming a plurality of corrected absorption signals,
• instructions for determining a plurality of tissue oxygenation measurements for the tissue of the subject based on the plurality of corrected absorption signals, and
• instructions for forming the two-dimensional image of tissue oxygenation from the plurality of corrected tissue oxygenation measurements.