Back to results
US 8,642,354 B2Granted
Fluid processing device for oligonucleotide synthesis and analysis
AssigneeApplied Biosystems, LLC
InventorSergey Ermakov
Life of this patent
Estimating the term…
Abstract
The present teachings provide a fluid processing device adapted to produce different oligomers in a plurality of respective reaction sites. The fluid processing device can comprise a first manifold for delivering reactants to the plurality of reaction sites, and a second manifold for removing waste from, and optionally delivering wash fluid to, the plurality of reaction sites. Surface tension control valves can be disposed in fluid communication with the first manifold and can selectively allow reactants and/or fluids into the reaction sites. A method of making oligonucleotides is also provided.
Claims10
A method comprising:
- introducing a fluid including a first monomer into a first fluid distribution manifold of a fluid processing device;
- opening, by a controller, a closed surface tension control valve in fluid communication with both the first fluid distribution manifold and at least one respective reaction site, to form an open surface tension control valve, wherein opening the closed surface tension control valve allows flow of the fluid to contact the at least one respective reaction site,
- wherein the surface tension control valve is configured to be actuated by electromagnetic radiation directed at the surface tension control valve to change a contact angle of the fluid to a surface of the surface tension control valve,
- wherein the at least one closed surface tension control valve is disposed between the first manifold and the at least one respective reaction site, and
- wherein the open surface tension control valve is configured to allow the first monomer to move from the first manifold, through the open surface tension control valve, and into the at least one respective reaction site;
- and
- forming an extended structure by the first monomer attaching to a first structure in the at least one respective reaction site.
4 more elements
Dependencies are inferred from the claim text.