Method and apparatus for pyrolysis-induced cleavage in peptides and proteins
AssigneeUniversity of Wyoming
InventorsFranco Basile, Shaofeng Zhang
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Abstract
A method and apparatus for conducting the rapid pyrolysis of peptides, proteins, polymers, and biological materials. The method can be carried out at atmospheric pressures and takes only about 5 to 30 seconds. The samples are cleaved at the C-terminus of aspartic acid. The apparatus employs a probe on which the sample is heated and digested components analyzed.
Claims6
A method of producing detecting non-volatile residues of a digested peptide sample containing aspartic acid and cysteine, comprising heating the peptide sample to between about 180° C. and about 250° C., in a period of between about 5 seconds and about 30 seconds to cleave the peptide at both the aspartic acid and cysteine site-specific locations to create at least one non-volatile residue, and detecting said non-volatile residue.
Dependencies are inferred from the claim text.