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US 8,637,283 B2Granted
Production of hydrocarbons in microorganisms
AssigneeMogene LC
InventorsAbhay Kumar Singh, Ganesh M. Kishore
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Abstract
A recombinant microorganism employing a bacterial pathway to produce a cyclic amino acid (e.g., coronamic acid or norcoronamic acid) and a plant enzyme (ACC oxidase) to oxidize the amino acid and produce an alkene (e.g., 1-butene or propene) is provided herein. Expression of these two biosynthetic modules in various microbial chassis will facilitate alkene production from diverse energy and carbon sources, including sugars, glycerol, CO2, CH4, H2, and sunlight.
Claims14
A recombinant prokaryotic microorganism of the genus Pseudomonas, comprising
- a) one or more coronamic acid biosynthesis genes whose expression results in production of coronamic and/or norcoronamic acid;
- and
- b) a gene encoding an 1-aminocyclopropane-1-carboxylic acid (ACC) oxidase, wherein at least one of said genes is a recombinant gene and said microorganism-produces 1-butene or propene.
Dependencies are inferred from the claim text.