Back to results
US 8,637,048 B2Granted
Immunizing compositions and methods of use
AssigneeEpitopix, LLC
InventorsDaryll A. Emery, Darren E. Straub
Life of this patent
Estimating the term…
Abstract
The present invention provides compositions including siderophore receptor polypeptides and porins from gram negative microbes, and preferably, lipopolysaccharide at a concentration of no greater than about 10.0 endotoxin units per milliliter. The present invention also provides methods of making and methods of using such compositions, including inducing the production of antibody in an animal.
Claims52
A composition comprising:
- an isolated whole cell preparation of gram negative microbes, wherein the gram negative microbes comprise:
- at least two siderophore receptor polypeptides (SRPs);
- and
- at least two porins;
- and
- a pharmaceutically acceptable carrier;
- wherein the gram negative microbes comprise members of the family Vibrionaceae.
4 more elements
A composition comprising:
- an isolated whole cell preparation of gram negative microbes, wherein the gram negative microbes comprise:
- at least two siderophore receptor polypeptides (SRPs);
- and
- at least two porins;
- and
- a pharmaceutically acceptable carrier:,
- wherein the gram negative microbes comprise Campylobacter spp.
4 more elements
A composition comprising:
- an isolated whole cell preparation of gram negative microbes, wherein the gram negative microbes comprise:
- at least two siderophore receptor polypeptides (SRPs);
- and
- at least two porins;
- and
- a pharmaceutically acceptable carrier;
- wherein the gram negative microbes comprise Actinobacillus spp., Haemophilus spp., Myxcobacteria spp., Sporocytophaga spp., Chondrococcus spp., Cytophaga spp., Flexibacter spp., Flavobacterium spp., Aeromonas spp., or any combination thereof.
4 more elements
A composition comprising:
- an isolated whole cell preparation of gram negative microbes, wherein the gram negative microbes comprise:
- at least two siderophore receptor polypeptides (SRPs) expressed by the gram negative microbe when the gram negative microbe is grown in the presence of 2,2-dipyridyl;
- and
- at least two porins;
- and
- a pharmaceutically acceptable carrier.
3 more elements
A composition comprising:
- an isolated whole cell preparation of gram negative microbes, wherein the gram negative microbes comprise:
- at least two siderophore receptor polypeptides (SRPs), wherein at least one SRP is not expressed by the gram negative microbe at a detectable level when the gram negative microbe is grown in the absence of 2,2-dipyridyl;
- and
- at least two porins;
- and
- a pharmaceutically acceptable carrier.
3 more elements
A composition comprising:
- whole cells comprising:
- a plurality of siderophore receptor polypeptides (SRPs) expressed by a gram negative microbe;
- and
- a plurality of porins expressed by the gram negative microbe;
- and
- a pharmaceutically acceptable carrier;
- wherein the gram negative microbes comprise members of the family Vibrionaceae.
4 more elements
A composition comprising:
- whole cells comprising:
- a plurality of siderophore receptor polypeptides (SRPs) expressed by a gram negative microbe;
- and
- a plurality of porins expressed by the gram negative microbe;
- and
- a pharmaceutically acceptable carrier;
- wherein the gram negative microbes comprise Campylobacter spp.
4 more elements
A composition comprising:
- whole cells comprising:
- a plurality of siderophore receptor polypeptides (SRPs) expressed by a gram negative microbe;
- and
- a plurality of porins expressed by the gram negative microbe;
- and
- a pharmaceutically acceptable carrier;
- wherein the gram negative microbes comprise Actinobacillus spp., Haemophilus spp., Myxcobacteria spp., Sporocytophaga spp., Chondrococcus spp., Cytophaga spp., Flexibacter spp., Flavobacterium spp., Aeromonas spp., or any combination thereof.
4 more elements
A composition comprising:
- whole cells comprising:
- a plurality of siderophore receptor polypeptides (SRPs) expressed by a gram negative microbe when the gram negative microbe is grown in the presence of 2,2-dipyridyl;
- and
- a plurality of porins expressed by the gram negative microbe;
- and
- a pharmaceutically acceptable carrier.
3 more elements
A composition comprising:
- whole cells comprising:
- a plurality of siderophore receptor polypeptides (SRPs) expressed by a gram negative microbe, wherein at least one SRP is not expressed by the gram negative microbe at a detectable level when the gram negative microbe is grown in the absence of 2,2-dipyridyl;
- and
- a plurality of porins expressed by the gram negative microbe;
- and
- a pharmaceutically acceptable carrier.
3 more elements
A composition comprising:
- an isolated whole cell preparation of gram negative microbes, wherein the gram negative microbes comprise:
- at least two siderophore receptor polypeptides (SRPs);
- and
- at least two porins;
- and
- a pharmaceutically acceptable carrier;
- wherein the gram negative microbe comprises an E. coli mutant lacking outer oligosaccharide side chains of LPS.
4 more elements
A composition comprising:
- whole cells comprising:
- a plurality of siderophore receptor polypeptides (SRPs) expressed by a gram negative microbe;
- and
- a plurality of porins expressed by the gram negative microbe;
- and
- a pharmaceutically acceptable carrier;
- wherein the gram negative microbe comprises an E. coli mutant lacking outer oligosaccharide side chains of LPS.
4 more elements
Dependencies are inferred from the claim text.