Method for isolating hepatitis A virus or spring viremia of carp virus
AssigneeIndustry Foundation of Chonnam National University
InventorsDu Woon Kim, Hee Min Lee, Se Young Cho, Sang Mu Ko, Kyung Seo Oh, Joseph Kwon, Jong Soon Choi
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Abstract
A method for isolating Hepatitis A virus or Spring viremia of Carp virus. A virus probe is prepared by linking a magnetic bead-conjugated Protein G with an anti-HAV (Hepatitis A Virus) antibody or an anti-rhabdovirus antibody. The virus probe is contacted with a sample to be analyzed to form a virus probe-virus complex. The virus probe-virus complex is then isolated. It may specifically isolate Hepatitis A virus or Spring viremia of Carp virus from a sample mixed viruses.
Claims6
A method for isolating Hepatitis A virus or Spring viremia of Carp virus, comprising:
- (a) preparing a virus probe by linking a magnetic bead-conjugated Protein G with an anti-HAV (Hepatitis A Virus) antibody or an anti-rhabdovirus antibody;
- (b) contacting a sample to be analyzed with the virus probe to form a virus probe-virus complex;
- (c) isolating the virus probe-virus complex;
- and
- (d) analyzing the virus of the virus probe-virus complex isolated in the step (c) by a gene amplification, wherein the gene amplification is carried out using a primer pair as set forth in SEQ ID NO:1 and SEQ ID NO:2 or a primer pair as set forth in SEQ ID NO:3 and SEQ ID NO:4 to detect Hepatitis A virus, or wherein the gene amplification is carried out using a primer pair as set forth in SEQ ID NO:5 and SEQ ID NO:6 to detect Spring viremia of Carp virus.
Dependencies are inferred from the claim text.