Sample substrate having a divided sample chamber and method of loading thereof
AssigneeApplied Biosystems, LLC
InventorDonald R. Sandell
Life of this patent
Estimating the term…
Abstract
A sample substrate configured for samples of biological material is provided. The sample substrate has a dual chambered sample well separated by a wall that may be punctured or otherwise breached to allow mixing of material contained in the two initially separate chambers. The chambers are connected by channels to fluid reservoirs, wherein the channels can be staked to prevent further fluid flow into and out of the chambers. Methods of loading a sample substrate are also provided.
Claims19
A sample substrate for biological samples, comprising:
- a first channel;
- a second channel distinct from the first channel;
- a sample chamber being defined by at least a first member and a second member;
- a first position in which the first member and the second member are fluidly isolated from one another, the first member is in fluid communication with the first channel, and the second member is in fluid communication with the second channel;
- and
- a second position in which the first channel is in fluid communication with the second channel;
- wherein the first member comprises a first wall and the second member comprises a second wall;
- and
- wherein the first member comprises a first chamber portion and the second member comprises a second chamber portion, the chamber portions being fluidly isolated from one another in the first position and the chamber portions being in fluid communication with one another in the second position.
A sample substrate for biological samples, comprising:
- a sample chamber comprising a first member and a second member;
- a first channel fluidly connected to the first member;
- a second channel fluidly connected to the second member;
- a first state in which the first channel and the second channel are fluidly isolated from one another;
- and
- a second state in which the first channel and the second channel are fluidly connected to one another.
A sample substrate for biological samples, comprising:
- a first fluid;
- a second fluid;
- a sample chamber comprising a wall portion, a first member, and a second member;
- a first state in which the members are fluidly isolated from one another;
- and
- a second state in which the members are fluidly connected to one another;
- wherein:
- in the first state, the wall portion and the first member define a first volume containing the first fluid, and the wall portion and the second member define a second volume containing the second fluid;
- and
- in the second state, the sample substrate is configured to fluidly mix the first fluid and the second fluid within the sample chamber.
A sample substrate for biological samples, comprising:
- a first channel;
- a second channel distinct from the first channel;
- a sample chamber being defined by at least a first member and a second member;
- a first position in which the first member and the second member are fluidly isolated from one another, the first member is in fluid communication with the first channel, and the second member is in fluid communication with the second channel;
- and
- a second position in which the first channel is in fluid communication with the second channel;
- wherein the first member comprises a first chamber portion and the second member comprises a second chamber portion, the chamber portions being fluidly isolated from one another in the first position and the chamber portions being in fluid communication with one another in the second position.
Dependencies are inferred from the claim text.