Dose surface method for determination of analyte ratios

A computer program product comprising a non-transitory computer readable medium storing a plurality of instructions for controlling a processor to perform an operation for obtaining a property of a biological sample, the instructions comprising:

• calculating a first concentration using a measured first response for a first analyte in a biological sample and a first regression function that maps the first response to the first concentration;
• receiving a second concentration for a second analyte of the biological sample
• receiving a non-linear function that maps the first and second concentrations to a proportion of the first and second concentrations in the biological sample;
• and
• calculating the proportion using the non-linear function and the first and second concentrations.

An apparatus for obtaining a property of a biological sample, the apparatus comprising:

• a measuring module capable of measuring responses of analytes in a biological sample;
• a memory to store the measured analyte responses from the measuring module;
• a non-transitory computer readable medium storing a plurality of instructions, the instructions comprising:
• calculating a first concentration using a measured first response of a first analyte and a first regression function that maps the first response to the first concentration;
• receiving a second concentration for a second analyte of the biological sample;
• receiving a non-linear function that maps the first and second concentrations to a proportion of the first and second concentrations in the biological sample;
• and
• calculating the proportion using the non-linear function and the first and second concentrations;
• and
• a processor to execute the computer readable instructions on the computer readable medium in order to calculate the proportion.

A method comprising:

• measuring a first response for a first analyte in a biological sample;
• calculating, by a computing system, a first concentration using the first response and a first regression function that maps the first response to the first concentration;
• receiving a proportion of the first analyte and the second analyte of the biological sample;
• receiving a second regression function that maps the first concentration and the proportion to a second concentration of the second analyte in the biological sample;
• and
• calculating, by the computing system, the second concentration using the second regression function, the first concentration, and the proportion.