Detection system and method
AssigneeKoninklijke Philips N.V.
InventorsReinhold Wimberger-Friedl, Jeroen Hans Nieuwenhuis, Johannes Joseph Hubertina Barbara Schleipen
Life of this patent
Estimating the term…
Abstract
A detection system combining an excitation radiation source providing excitation radiation to an analysis region of a sample within a substrate having a detection surface, a detector for detecting radiation collected from the analysis region comprising the detection surface of the sample resulting from the excitation, and a magnet arrangement beneath the analysis region of the sample, and stationary with respect to the excitation radiation source and light coupling arrangement, for attracting magnetic beads within the sample to the substrate surface. The detection radiation is collected from the detection surface of the substrate to give an enhanced surface specificity.
Claims20
A detection system comprising:
- a substrate having a detection surface for holding a sample and including an excitation region at least partly in contact with the sample, and a radiation coupling arrangement for advancing excitation radiation from an excitation radiation source to the citation region to interact with bound labels of the sample to produce light coming from the bound labels;
- a collection arrangement positioned in the excitation region for collecting and directing the light coming from the bound labels;
- a detector for detecting the collected light;
- and
- a magnet arrangement configured to be disposed next to and at the same side of the detection surface, to be stationary with respect to the excitation radiation source and the radiation coupling arrangement, and to attract magnetic beads within the sample to the detection surface.
A detection method comprising acts of:
- disposing a magnet arrangement next to and at the same side of a detection surface of a substrate, the detection surface holding a sample and including an excitation region at least partly in contact with the sample;
- advancing excitation radiation from an excitation radiation source to the excitation region using a radiation coupling arrangement to interact with bound labels of the sample producing light coming from the bound labels;
- collecting and directing radiation of the light coming from the bound labels using a collection arrangement positioned in the excitation region;
- and
- detecting the collected light coming from the bound labels.
A detection system comprising:
- a substrate having a detection surface for holding a sample and including an excitation region in contact with the sample, and a radiation coupling configured to advance excitation radiation from an excitation radiation source to the excitation region to interact with bound labels of the sample to produce light coming from the bound labels;
- a collector positioned in the excitation region configured to collect and direct the light coming from the bound labels;
- a magnet arrangement configured to be disposed next to and at the same side of the detection surface, to be stationary with respect to the excitation radiation source and the radiation coupling arrangement, and to attract magnetic beads within the sample to the detection surface;
- and
- a detector, configured to detect the light coming from the bound labels, mounted on the surface of the magnet arrangement that is closest to the sample holder.
Dependencies are inferred from the claim text.