Transgenic maize event MON 87427 and the relative development scale

A DNA probe comprising a nucleotide sequence of sufficient length of contiguous nucleotides of SEQ ID NO:

• 10, or a full complement thereof, to function as a DNA probe that hybridizes under stringent hybridization conditions with a DNA molecule comprising a nucleotide sequence selected from the group consisting of SEQ ID NO:
• 1-8 and SEQ ID NO:
• 10 and does not hybridize under the stringent hybridization conditions with a DNA molecule not comprising a nucleotide sequence selected from the group consisting of SEQ ID NO:
• 1-8 and SEQ ID NO:
• 10, and wherein said DNA probe comprises the nucleotide sequence of SEQ ID NO:
• 1 or SEQ ID NO:
• 2.

A pair of DNA molecules comprising a first DNA molecule and a second DNA molecule different from the first DNA molecule, wherein said first and second DNA molecules each comprise a nucleotide sequence of sufficient length of contiguous nucleotides of SEQ ID NO:

• 10, or a full complement thereof, and wherein said first DNA molecule resides in a transgene insert DNA sequence of SEQ ID NO:
• 10, and said second DNA molecule resides in the maize genomic DNA sequence of SEQ ID NO:
• 10, to function as DNA primers when used together in an amplification reaction with DNA from event MON 87427 to produce an amplicon diagnostic for transgenic maize event MON 87427 DNA in a sample, and wherein said amplicon comprises the nucleotide sequences of SEQ ID NO:
• 1 or SEQ ID NO:
• 2.

A DNA kit comprising:

• a) a pair of DNA molecules comprising a first DNA molecule and a second DNA molecule different from the first DNA molecule, wherein said first and second DNA molecules each comprise a nucleotide sequence of sufficient length of contiguous nucleotides of SEQ ID NO:
• 10, or a full complement thereof, and wherein said first DNA molecule resides in a transgene insert DNA sequence of SEQ ID NO:
• 10, and said second DNA molecule resides in the maize genomic DNA sequence of SEQ ID NO:
• 10, to function as DNA primers when used together in an amplification reaction with DNA from event MON 87427 to produce an amplicon diagnostic for transgenic maize event MON 87427 DNA in a sample, and wherein said amplicon comprises the nucleotide sequences of SEQ ID NO:
• 1 or SEQ ID NO:
• 2;
• or
• b) at least one DNA probe diagnostic for event MON 87427, wherein the DNA probe comprises the nucleotide sequence of SEQ ID NO:
• 1 or SEQ ID NO:
• 2.

A method of producing a maize plant that tolerates application of glyphosate herbicide comprising:

• a) sexually crossing a transgenic maize plant comprising event MON 87427 comprising a nucleic acid molecule comprising a nucleotide sequence selected from the group consisting of SEQ ID NO:
• 10 with a second maize plant, thereby producing seed;
• b) collecting said seed produced from said cross;
• c) growing said seed to produce a plurality of progeny plants;
• d) treating said progeny plants with glyphosate;
• and
• e) selecting a progeny plant that is tolerant to glyphosate.

A method of producing a maize plant that tolerates application of glyphosate herbicide comprising:

• a) selfing a transgenic maize plant comprising event MON 87427 comprising a nucleic acid molecule comprising a nucleotide sequence selected from the group consisting of SEQ ID NO:
• 1-8 and SEQ ID NO:
• 10, thereby producing seed;
• b) collecting said seed produced from said selfing;
• c) growing said seed to produce a plurality of progeny plants;
• d) treating said progeny plants with glyphosate;
• and
• e) selecting a progeny plant that is tolerant to glyphosate.

A method of producing hybrid maize seed comprising:

• a) planting transgenic maize seed comprising event MON 87427 in an area;
• b) growing a maize plant from said seed;
• c) treating said plant with an effective dose of glyphosate herbicide prior to pollen formation to make said plant male sterile without injuring said plant;
• d) fertilizing said plant with pollen from a second parent plant;
• and
• e) harvesting seed from said plant, wherein said seed is hybrid maize seed produced by the cross of a transgenic maize plant comprising event MON 87427 with a second parent plant.