Polysaccharide synthases (H)
OwnerMonika Susanne Doblin, Filomena Angela Pettolino +4inventors
InventorsMonika Susanne Doblin, Filomena Angela Pettolino, Antony Bacic, Stephen Alan Jobling, Geoffrey Bruce Fincher, Rachel Anita Burton
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Abstract
The present invention relates generally to polysaccharide synthases. More particularly, the present invention relates to (1,3;1,4)-β-D-glucan synthases. The present invention provides, among other things, methods for influencing the level of (1,3;1,4)-β-D-glucan produced by a cell and nucleic acid and amino acid sequences which encode (1,3;1,4)-β-D-glucan synthases.
Claims15
A method for modulating the level of (1,3;1,4)-β-D-glucan produced by a plant or fungal cell, the method comprising modulating the level and/or activity of a CslH-encoded (1,3;1,4)-β-D-glucan synthase in the cell, wherein the level and/or activity of the CslH-encoded (1,3;1,4)-β-D-glucan synthase is modulated by modulating the expression of a CslH nucleic acid in the cell, and wherein modulating the expression of a CslH nucleic acid in the cell results in modulation of the level of (1,3;1,4)-β-D-glucan produced by the cell compared to a wild-type cell of the same taxon, and wherein the CslH nucleic acid comprises:
- (i) a nucleotide sequence set forth in SEQ ID NO:
- 1;
- (ii) a nucleotide sequence encoding the amino acid sequence set forth in SEQ ID NO:
- 2;
- or
- (iii) a nucleotide sequence encoding an amino acid sequence which is at least 95% identical to the amino acid sequence set forth in SEQ ID NO:
- 2.
A plant or fungal cell comprising any one or more of:
- a modulated level and/or activity of CslH-encoded (1,3;1,4)-β-D-glucan synthase relative to a wild type cell of the same taxon;
- and/or
- modulated expression of a CslH nucleic acid relative to a wild type cell of the same taxon,
- wherein the cell comprises a modulated level of (1,3;1,4)-β-D-glucan relative to a wild-type cell of the same taxon,
- wherein the CslH nucleic acid comprises:
- (i) a nucleotide sequence set forth in SEQ ID NO:
- 1;
- (ii) a nucleotide sequence encoding the amino acid sequence set forth in SEQ ID NO:
- 2;
- or
- (iii) a nucleotide sequence encoding an amino acid sequence which is at least 95% identical to the amino acid sequence set forth in SEQ ID NO:
- 2.
Dependencies are inferred from the claim text.