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US 8,618,353 B2Granted
Lolium multiflorum line inducing genome loss
AssigneeThe United States of America as represented by the Secretary of Agriculture
InventorBryan K. Kindiger
Life of this patent
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Abstract
Dihaploid homozygous Fescue species may be produced by interspecific hybridization of Fescue with novel ryegrass, Lolium multiflorus, lines, that induce genome loss. Plants that are dihaploid homozygous Lolium and amphidiploids are also produced.
Claims28
A method for producing fertile dihaploid homozygous Festuca species, comprising:
- a) providing a Lolium multiflorum diploid line which segregates for levels of pollen sterility and where upon using the Lolium as the maternal parent in an interspecific hybridization with a Festuca species, being utilized as the paternal parent, confers to the resultant sterile F1 interspecific hybrids the ability to produce some seed via parthenogenesis that also possess a viable embryo, wherein said seeds which possess a viable embryo may develop into F2 generation plants at least some of which are dihaploid homozygous Festuca species comprising a karyotype exhibiting a balanced genome of Festuca chromosomes equivalent to the chromosome number of said Festuca paternal parent and no Lolium chromosomes;
- b) crossing said Lolium multiflorum diploid line as the maternal parent with a Festuca species utilized as the paternal parent to generate sterile F1 interspecific hybrid plants;
- c) recovering and planting the panicle obtained from said F1 interspecific hybrid plants, wherein those of said seed heads that have developed embryos via parthenogenesis will develop into F2 plants;
- and
- d) growing said F2 plants, wherein at least some of said F2 plants comprise dihaploid homozygous Festuca species comprising a karyotype with a balanced genome of Festuca chromosomes equivalent to the chromosome number of said Festuca paternal parent and no Lolium chromosomes, and further comprising extranuclear genes of Lolium multiflorum;
- wherein said Lolium multiflorum diploid line used as the maternal parent is selected from the group consisting of IL-1 (ATCC deposit accession no. PTA-10229) and progeny thereof which comprise L. multiflorum extranuclear genes consisting of the extranuclear genes of IL-1;
- or
- wherein said Lolium multiflorum diploid line used as the maternal parent is selected from the group consisting of IL-2 (ATCC deposit accession no. PTA-10315) and progeny thereof which comprise L. multiflorum extranuclear genes consisting of the extranuclear genes of IL-2.
5 more elements
A method for producing fertile dihaploid homozygous Festuca species, comprising:
- a) providing a Lolium multiflorum diploid line which segregates for levels of pollen sterility and where upon using the Lolium as the maternal parent in an interspecific hybridization with a Festuca species, being utilized as the paternal parent, confers to the resultant sterile F1 interspecific hybrids the ability to produce some seed via parthenogenesis that also possess a viable embryo, wherein said seeds which possess a viable embryo may develop into F2 generation plants at least some of which are dihaploid homozygous Festuca species comprising a karyotype exhibiting a balanced genome of Festuca chromosomes equivalent to the chromosome number of said Festuca paternal parent and no Lolium chromosomes;
- b) crossing said Lolium multiflorum diploid line as a maternal parent with a Festuca species as the paternal parent to generate sterile F1 interspecific hybrid plants;
- c) identifying somatic sectors in said F1 plants or in plants which are progeny thereof in which somatic sectors appear as fescue growing shoots comprising a dihaploid homozygous Festuca karyotype with all Festuca chromosomes and no Lolium chromosomes;
- and
- d) retaining said F1 plants or said progeny in (c) which are identified as possessing said somatic sectors or recovering said somatic sectors therefrom;
- wherein said somatic sectors develop into mature plants comprising dihaploid homozygous Festuca species comprising a karyotype exhibiting a balanced genome of Festuca chromosomes equivalent to the chromosome number of said Festuca paternal parent and no Lolium chromosomes, and further comprising extranuclear genes of Lolium multiflorum;
- wherein said Lolium multiflorum diploid line used as the maternal parent is selected from the group consisting of IL-1 (ATCC deposit accession no. PTA-10229) and progeny thereof which comprise L. multiflorum extranuclear genes consisting of the extranuclear genes of IL-1;
- or
- wherein said Lolium multiflorum diploid line used as the maternal parent is selected from the group consisting of IL-2 (ATCC deposit accession no. PTA-10315) and progeny thereof which comprise L. multiflorum extranuclear genes consisting of the extranuclear genes of IL-2.
6 more elements
Dependencies are inferred from the claim text.