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US 8,618,261 B2Granted
Immuno-based botulinum toxin serotype A activity assays
AssigneeAllergan, Inc.
InventorsFernandez-Salas Ester, Joanne Wang, Patton Garay, Lina S. Wong, D. Dianne Hodges, Kei Roger Aoki
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Abstract
The present specification discloses SNAP-25 compositions, methods of making α-SNAP-25 antibodies that bind an epitope comprising a carboxyl-terminus at the P1 residue from the BoNT/A cleavage site scissile bond from a SNAP-25 cleavage product, α-SNAP-25 antibodies that bind an epitope comprising a carboxyl-terminus at the P1 residue from the BoNT/A cleavage site scissile bond from a SNAP-25 cleavage product, methods of detecting BoNT/A activity, and methods of detecting neutralizing α-BoNT/A antibodies.
Claims2
An isolated α-SNAP-25 antibody,
- wherein the isolated α-SNAP-25 antibody specifically binds to the peptide of SEQ ID NO:38;
- wherein said antibody specifically binds to an epitope of said fragment of said SNAP25 polypeptide comprising the C-terminal amino acid sequence of SEQ ID NO:38 with an equilibrium disassociation constant of less than 0.450 nM;
- and,
- wherein said antibody has an association rate constant for an epitope of intact SNAP-25 polypeptide comprising SEQ ID NO:5 of less than 1×101 M−1 s−1.
1 more element
Dependencies are inferred from the claim text.