Insulin analogues of prolonged activity

Insulin derivative or its pharmaceutically acceptable salt containing two polypeptides forming chain A and chain B, characterised by being a new polipeptide of the formula 1:

• wherein R denotes group of formula Asn-R2, where R2 denotes neutral L-amino acid;
• and R1 denotes B31Lys-B32Arg, wherein B3Asn can be optionally replaced by B3Glu, or B31Arg, wherein amino acid sequence of chain A has been chosen from SEQ ID NO:
• 1-3, while amino acid sequence of chain B has been chosen from SEQ ID NO:
• 7, 9, and 10 in one of the following combinations:
• SEQ ID NO:
• 1 with SEQ ID NO:
• 7, SEQ ID NO:
• 1 with SEQ ID NO:
• 9, SEQ ID NO:
• 1 with SEQ ID NO:
• 10, SEQ ID NO:
• 2 with SEQ ID NO:
• 7, SEQ ID NO:
• 3 with SEQ ID NO:
• 7, SEQ ID NO:
• 3 with SEQ ID NO:
• 10,
• and wherein the new polypeptide of formula 1 is an analogue of recombined human insulin of isoelectric point 5-8.5.