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US 8,617,897 B2Granted
Method of detecting oxides of nitrogen
Assignee3M Innovative Properties Company
InventorVinod P. Menon
Life of this patent
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Abstract
Methods and kits to detect and quantitate NOx compounds in a biological sample are provided. The methods include reacting a mixture that includes a nitrite compound and a chromogenic reagent to form a colored compound, contacting the mixture with a retention medium configured to retain the colored compound, and detecting the colored compound retained on the retention medium.
Claims11
A method of detecting a NOx compound in a wound, comprising:
- providing a sample from a wound, a first retention medium, and a chromogenic reagent;
- wherein the chromogenic reagent is capable of reacting with a nitrite compound to form a colored compound;
- wherein the first retention medium is configured to retain the colored compound
- forming a first mixture comprising the sample and the chromogenic reagent under conditions that permit the reaction of a nitrite compound with the chromogenic reagent to form the colored compound;
- heating the first mixture;
- diluting the first mixture with an aqueous liquid;
- contacting at least a portion of the first mixture with the first retention medium;
- and
- detecting the colored compound retained on the first retention medium;
- wherein the first retention medium comprises a medium with ionic functional groups that interact with the colored compound;
- wherein the contacting at least a portion of the first mixture with the first retention medium occurs after the heating the first mixture and after the diluting the first mixture with an aqueous liquid.
8 more elements
Dependencies are inferred from the claim text.