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US 8,617,892 B2Granted
Microbubble devices, methods and systems
AssigneeThe Trustees of Columbia University in the City of New York
InventorsMark A. Borden, Eric G. Lima, Clark T. Hung, Shashank Ramesh Sirsi
Life of this patent
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Abstract
A hydrogel tissue engineering scaffold having microbubbles dispersed therein is disclosed. Also, a system for cell culturing including a controller and actuator to apply dynamic deformational loading to a hydrogel is disclosed. Also disclosed are methods for producing hydrogels with microbubbles and for culturing cells using hydrogels with microbubbles.
Claims14
A method of mechanically stimulating cells in a hydrogel, the method comprising:
- incorporating gas-filled microbubbles into the hydrogel;
- seeding the hydrogel with cells;
- and
- compressing the hydrogel with the gas-filled microbubbles incorporated therein so as to produce a non-zero divergence of a volume in a portion of the hydrogel during a culturing cycle.
1 more element
A method comprising:
- incorporating gas-filled microbubbles into a hydrogel;
- seeding the hydrogel with cells;
- and
- isotropically compressing the hydrogel with the gas-filled microbubbles incorporated therein by applying a hydrostatic loading during a culturing cycle of the cells seeded in the hydrogel so as to produce a non-zero divergence of a volume in a portion of the hydrogel.
1 more element
Dependencies are inferred from the claim text.