Methods and compositions for the repair and/or regeneration of damaged myocardium using cytokines and variants thereof
AssigneeNew York Medical College
InventorPiero Anversa
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Abstract
Methods, compositions, and kits for repairing damaged myocardium and/or myocardial cells including the administration of cytokines, variants of cytokines, cardiac stem cells, or combinations thereof are disclosed and claimed. In addition, methods, compositions, and kits for forming coronary vasculature including the administration of cytokines, variants of cytokines, cardiac stem cells, or combinations thereof are described. In particular, administration of variants of hepatocyte growth factor, such as NK1, 1K1, and HP11, are useful for the repair and/or regeneration of damaged myocardium or formation of coronary vasculature. Methods of activating cardiac stem cells in vitro are also disclosed.
Claims22
A method of activating adult cardiac stern cells comprising incubating isolated adult cardiac stem cells in a solution comprising at least one cytokine, wherein the at least one cytokine is a variant of hepatocyte growth factor selected from the group consisting of NK1, 1K1, K2, HP11, HP 12, and HP21.
A method for restoring structural and functional integrity to damaged myocardium in a subject in need thereof comprising:
- extracting cardiac stem cells from the subject;
- culturing and expanding said cardiac stem cells;
- activating the extracted and expanded cardiac stem cells by exposing the extracted and expanded cardiac stem cells to a variant of hepatocyte growth factor selected from the group consisting of NK1, 1K1, 1K2, HP11, HP12, and HP21;
- and
- administering an effective dose of said activated cardiac stem cells to an area of damaged myocardium the subject, wherein the activated cardiac stem cells restore structural and functional integrity to the damaged myocardium following their administration.
Dependencies are inferred from the claim text.