Bacterial mediated delivery of nuclear protein into pluripotent and differentiated cells
AssigneeUniversity of Florida Research Foundation, Inc.
InventorsShouguang Jin, Candace Bichsel
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Abstract
A modified P. aeruginosa type III secretion system has been developed that efficiently delivers selected proteins into a host cell. In one example, a functional nuclear Cre Recombinase is injected into embryonic stem (ES) cells and can be used to induce pluripotent stem (iPS) cells. This method of in vitro lineage directed differentiation prevents insertional mutagenesis and provides a route to selected stem cell renewal and cell-based therapies.
Claims15
A method of delivering one or more nuclear proteins into one or more isolated cells, comprising:
- incubating the cell or cells with a Pseudomonas bacteria deletion mutant lacking genes encoding ExoS, ExoT, and ExoY proteins, said deletion mutant comprising a plasmid or plasmids comprising nucleic acid sequences encoding one or more fusion proteins, wherein each nucleic acid sequence comprises a nucleic acid sequence encoding a nuclear protein fused to a nucleic acid sequence encoding the N-terminus of an ExoS protein or a nucleic acid sequence encoding a signal sequence from an ExoS protein, and the nucleic acid further comprises a nucleic acid sequence encoding a nuclear localization sequence between the nucleic acid sequence encoding the N-terminus of an ExoS protein or a nucleic acid sequence encoding a signal sequence from an ExoS protein and the nucleic acid sequence encoding the nuclear protein;
- and incubating the isolated infected cell or cells for a period of time sufficient to deliver the one or more nuclear proteins into said cell or cells.
A method for inducing one or more isolated differentiated cell or cells into pluripotent stem (iPS) cell or cells, comprising:
- infecting the isolated differentiated cell or cells with a Pseudomonas bacteria deletion mutant lacking genes encoding ExoS, ExoT, and ExoY proteins, said deletion mutant comprising a plasmid or plasmids comprising nucleic acid sequences encoding one or more fusion proteins, wherein each nucleic acid sequence comprises a nucleic acid sequence encoding a stem cell specific transcriptional factor fused to a nucleic acid sequence encoding the N-terminus of an ExoS protein or a nucleic acid sequence encoding a signal sequence from an ExoS protein, and the nucleic acid further comprises a nucleic acid sequence encoding a nuclear localization sequence between the nucleic acid sequence encoding the N-terminus of an ExoS protein or a nucleic acid sequence encoding a signal sequence from an ExoS protein and the nucleic acid sequence encoding the transcriptional factors, wherein the transcription factors are Oct4, Sox2, c-Myc, and Klf4;
- incubating the isolated infected cell or cells under embryonic stem (ES) cell culture conditions such that Oct-4, Sox-2, c-Myc and Klf-4 proteins are introduced into the cell or cells to form a population of iPS cells.
A method of delivering one or more nuclear proteins into one or more isolated cells, comprising:
- incubating the cell or cells with a Pseudomonas aerogenosa bacteria deletion mutant lacking genes encoding ExoS, ExoT, and ExoY proteins, said deletion mutant comprising a plasmid or plasmids comprising nucleic acid sequences encoding one or more fusion proteins, wherein each nucleic acid sequence comprises a nucleic acid sequence encoding a nuclear protein fused to a nucleic acid sequence encoding the N-terminus of an ExoS protein or a nucleic acid sequence encoding a signal sequence from an ExoS protein, and the nucleic acid further comprises a nucleic acid sequence encoding a nuclear localization sequence between the nucleic acid sequence encoding the N-terminus of an ExoS protein or a nucleic acid sequence encoding a signal sequence from an ExoS protein and the nucleic acid sequence encoding the nuclear protein;
- and incubating the isolated infected cell or cells for a period of time sufficient to deliver the one or more nuclear proteins into said cell or cells.
Dependencies are inferred from the claim text.