Cultured hematopoietic stem cells and method for expansion and analysis thereof
AssigneeWhitehead Institute for Biomedical Research
InventorsChengcheng Zhang, Harvey Lodish
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Abstract
Hematopoietic stem cells and methods for ex vivo expansion of hematopoietic stem cells are provided. The methods comprise culturing the cells in a media containing an effective amount insulin-like growth factor(IGF), fibroblast growth factor (FGF), thrombopoietin (TPO), and stem cell factor (SCF), under conditions sufficient for expansion of said cells. Methods for identifying expanded hematopoeitc stem cells and kits for ex vivo expansion of hematopoietic stem cells are also provided.
Claims17
A method for expanding hematopoietic stem cells ex vivo, comprising culturing a population of cells that includes hematopoietic stem cells in a medium comprising insulin-like growth factor 2 (IGF-2) and stem cell factor (SCF), under conditions sufficient for expansion of the hematopoietic stem cells, thereby expanding hematopoietic stem cells ex vivo and enriching for hematopoietic stem cells relative to the initial population of cells.
A kit for expanding and enriching for hematopoietic stem cells ex vivo comprising a) insulin-like growth factor 2 (IGF-2), stem cell factor (SCF), thrombopoietin (TPO), and fibroblast growth factor 1 (FGF-1);
- and b) instructions for expanding hematopoietic stem cells ex vivo and for enriching for hematopoietic stem cells relative to an initial population of cells that includes hematopoietic stem cells.
A method for expanding hematopoietic stem cells ex vivo, comprising culturing a population of cells that includes hematopoietic stem cells in a serum-free medium comprising insulin-like growth factor-2 (IGF-2), fibroblast growth factor 1 (FGF-1), thrombopoietin (TPO), and stem cell factor (SCF), under conditions sufficient for expansion of the hematopoietic stem cells, thereby expanding hematopoietic stem cells ex vivo.
Dependencies are inferred from the claim text.