Methods for eliminating at least a substantial portion of a clonal antigen-specific memory T cell subpopulation
AssigneeLife Technologies Corporation
InventorsRonald J Berenson, Mark Bonyhadi, Dale Kalamasz
Life of this patent
Estimating the term…
Abstract
The present invention relates generally to methods for stimulating T cells, and more particularly, to methods to eliminate undesired (e.g., autoreactive, alloreactive, pathogenic) subpopulations of T cells from a mixed population of T cells, thereby restoring the normal immune repertoire of said T cells. The present invention also relates to compositions of cells, including stimulated T cells having restored immune repertoire and uses thereof.
Claims6
A method for eliminating at least a substantial portion of a clonal antigen-specific memory T cell subpopulation from a mixed population of T cells from an individual, comprising,
- exposing a population of cells comprising the mixed population of T cells to a surface,
- said exposure comprising contacting said population of cells with said surface at a ratio of surface to cells of at least 5:1, wherein the surface has attached thereto an anti-CD3 antibody and an anti-CD28 antibody, or antigen-binding fragments thereof;
- and wherein said exposure results in apoptosis or growth inhibition in at least a substantial portion of at least one clonal antigen-specific memory T cell population present in the mixed population of T cells without requiring further activation or stimulation;
- thereby eliminating at least a substantial portion of said clonal antigen-specific memory T cell population from the mixed population of T cells.
Dependencies are inferred from the claim text.