Back to results
US 8,617,881 B2Granted
Methods for producing recombinant proteins
AssigneeExcelimmune, Inc.
InventorsVincent W. Coljee, Gwendolyn M. Wilmes, Stuart Hicks, Kimberly L. Carey, Elizabeth R. Reczek
Life of this patent
Estimating the term…
Abstract
Methods for producing recombinant cell populations are disclosed. The disclosed methods may be used to produce therapeutic polyclonal proteins.
Claims27
A method for producing a polyclonal cell population, said method comprising the steps of:
- a) transforming one or more host cells with at least one vector comprising at least one copy of a nucleic acid sequence that encodes a component of a polyclonal protein composition, in the presence of a recombinase or integrase, such that the at least one vector integrates into the host cell genome at a plurality of locations recognized by the recombinase or integrase;
- b) generating a cell population, without clonal selection, capable of producing the component of the polyclonal protein composition;
- and
- c) mixing the cell population and a different cell population producing a different component of the polyclonal protein composition generated by performing steps (a) and (b), each cell population comprising greater than about 10,000 individual cells to produce a polyclonal cell population capable of producing the polyclonal protein composition.
1 more element
A method for producing a polyclonal cell population, said method comprising the steps of:
- a) transforming one or more host cells with at least one adeno-associated viral (AAV) vector comprising at least one copy of a nucleic acid sequence, in the presence of an AAV rep protein, such that the at least one AAV vector integrates into the host cell genome at a plurality of locations recognized by the AAV rep protein and that encodes a component of a polyclonal protein composition;
- b) generating without clonal selection a cell population capable of producing the component of the polyclonal protein composition;
- and
- c) mixing the cell population and a different cell population capable of producing a different component of the polyclonal protein composition generated by performing steps (a) and (b) to produce a polyclonal cell population capable of producing the polyclonal protein composition.
1 more element
Dependencies are inferred from the claim text.