Chimeric toll-like receptor/MD-2 polypeptides and methods of use thereof
AssigneeNovImmune S.A.
InventorGreg Elson
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Abstract
This invention provides monoclonal antibodies that recognize the Toll-like Receptor 4/MD-2 receptor complex, and monoclonal antibodies that recognize the TLR4/MD2 complex as well as TLR4 when not complexed with MD-2. The invention further provides methods of using the monoclonal antibodies as therapeutics. This invention also provides soluble chimeric proteins, methods of expressing and purifying soluble chimeric proteins, and methods of using soluble chimeric proteins as therapeutics, in screening assays and in the production of antibodies.
Claims6
A vector comprising a nucleic acid sequence encoding a TLR4 polypeptide coupled to a nucleic acid sequence encoding an MD-2 accessory polypeptide, wherein the toll-like receptor polypeptide comprises at least a fragment of the extracellular portion of the toll-like receptor, and wherein the toll-like receptor polypeptide is operably linked to the MD accessory polypeptide by means of a glycine-serine linker.
An isolated chimeric polypeptide comprising a toll-like receptor polypeptide operably linked to an MD accessory polypeptide by means of a glycine-serine linker, wherein the toll-like receptor polypeptide comprises at least a fragment of the extracellular portion of the toll-like receptor.
Dependencies are inferred from the claim text.