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US 8,617,871 B2Granted
Method and culture medium for enhanced detection of Mycobacterium
AssigneeBiomerieux
InventorsParampal Deol, Leticia Barton, Yoany Portilla, Douglas Lovern
Life of this patent
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Abstract
The present invention relates to an improved culture medium and method for the enhanced growth and detection of Mycobacterium growth. The invention further relates to an improved mycobacterial reagent system or kit that can be used for the enhanced growth and detection of Mycobacterium.
Claims20
A method for the diagnosis of infection caused by a mycobacterium species, comprising the steps of:
- (a) providing a base mycobacterial culture medium;
- (b) adding a nutrient supplement containing α-ketoglutarate to said culture medium so as to provide a final concentration of at least about 5 g/L of α-ketoglutarate to the culture medium;
- (c) adding an effective amount of an antimicrobial supplement to said culture medium to suppress the growth of contaminating respiratory flora;
- (d) adding a sample for which the presence or absence of said mycobacterium species is to be determined;
- and (e) analyzing said culture medium for the presence of said mycobacterium species, wherein a finding of the presence of said mycobacterium species indicates a positive diagnosis for said infection.
2 more elements
A kit for detecting the growth of mycobacteria in a culture medium, said kit comprising the following components separately housed therein:
- (1) a culture bottle containing a base mycobacterial culture medium;
- (2) a nutrient supplement comprising α-ketoglutarate, wherein the α-ketoglutarate is in an amount sufficient to supply a final concentration of at least about 5 g/L of α-ketoglutarate to the culture medium;
- and (3) an antimicrobial supplement for suppressing the growth of contaminating respiratory flora in said culture medium.
Dependencies are inferred from the claim text.