Asparaginase enzyme variants and uses thereof
AssigneeDsm IP Assets B.V.
InventorsJan Metske Van Der Laan, Mark Cristiaan Stor, Ilse Lange De, Lisette Mohrmann
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Abstract
The present invention relates to newly identified asparaginase polypeptide variants of SEQ ID NO: 3 and to polynucleotide sequences that encode such novel asparaginase variants. Furthermore the invention relates to the use of these novel asparaginase variants in industrial processes.
Claims29
A variant of a parent polypeptide having asparaginase activity, wherein the variant has an amino acid sequence which, when aligned with the sequence set out in SEQ ID NO:
- 3, comprises substitution of an amino acid residue corresponding to any of amino acids 41, 62, 63, 76, 77, 101, 104, 106, 108, 119, 122, 123, 132, 140, 142, 143, 145, 162, 163, 169, 170, 195, 213, 217, 218, 232, 233, 234, 268, 269, 270, 272, 273, 293, 297, 298, 300, 301, 304, or 371;
- said positions being defined with reference to SEQ ID NO:
- 3, and wherein the parent polypeptide has at least 90% homology with SEQ ID NO:
- 3.
A variant of a parent polypeptide having asparaginase activity, wherein the variant has an amino acid sequence which, when aligned with the sequence set out in SEQ ID NO:
- 3, comprises one or more of:
- Tyr at position 53, Pro at position 64, Lys at position 73, Ala at position 74, Thr at position 76, Ile at position 77, Tyr or Pro at position 88, Val at position 90, Glu at position 91, Gly at position 111, Asp at position 195, Ser at position 211, His at position 228, Cys or His at position 262, Tyr at position 267, Ser at position 295, Ser at position 299, Ser at position 303, Val at position 310, Asp at position 314, Ile at position 317, Ala at position 319, Thr at position 321, Gly at position 324, Ser at position 330, Ala at position 332, or Glu at position 333;
- said positions being defined with reference to SEQ ID NO:
- 3, and wherein the parent polypeptide has at least 90% homology with SEQ ID NO:
- 3.
Dependencies are inferred from the claim text.