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US 8,617,865 B2Granted
Tannase, gene encoding same, and process for producing same
AssigneeAmano Enzyme Inc.
InventorsMegumi Nakagawa, Naoki Matsumoto, Hitoshi Amano, Shotaro Yamaguchi
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Abstract
Disclosed is a thermostable tannase derived from a microorganism. Specifically disclosed is a thermostable tannase derived from Aspergillus awamori or Aspergillus niger. A preferred embodiment of the tannase has the following chemoenzymatic properties: (1) activity: to act on a depside bond to thereby cause hydrolysis; (2) molecular weight: about 230,000 Da (as measured by gel filtration); and (3) thermal stability: stable at a temperature up to 65° C. (pH 5.0, 30 min.)
Claims8
An isolated tannase having the amino acid sequence set forth in SEQ ID NO:
- 5 or an amino acid sequence that is 90% or more identical to the amino acid sequence set forth in SEQ ID NO:
- 5 and having the following chemoenzymatic properties:
- (1) activity:
- to act on a depside bond to thereby cause hydrolysis;
- (2) molecular weight:
- about 230,000 Da (as measured by gel filtration);
- and
- (3) thermostability:
- stable at a temperature up to 65° C. (pH 5.0, 30 min.).
6 more elements
Dependencies are inferred from the claim text.