Oligonucleotides for detecting chicken astrovirus
AssigneeAgri-Food Biosciences Institute
InventorsDaniel Todd, Victoria Jane Smyth
Life of this patent
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Abstract
There is provided an oligonucleotide sequence capable of binding to a portion of a CAstV genome, wherein the oligonucleotide sequence has binding specificity to the precapsid region of CAstV or to cDNA of the precapsid region. The oligonucleotide sequence can be one of a primer pair for use in a method for detecting the presence of CAstV in a biological sample by reverse transcription followed by amplification of the reverse transcription products using such primer pair, or a method for amplifying CAstV cDNA using such primer pair.
Claims7
A primer pair capable of amplifying a portion of a CAstV genome, wherein the primer pair comprises at least one oligonucleotide primer sequence that has binding specificity to a precapsid region of the CAstV genome;
- wherein the precapsid region is defined as a 106 nucleotide region located at the 3′ end of the polymerase gene, wherein the 106 nucleotide region comprises an intergenic region between the polymerase gene (orf1b) and the capsid gene (orf2) and the first four nucleotides of the capsid gene, and wherein the precapsid region has a polynucleotide sequence
- TGCGTCGAGGAGTATGCCGCTGCTGAAGAAATACAGTTACCAGAAGTCGGGC CCGACTTCTTTCAGAAAATCTGGTAGAGGGATGGACCGAAATATAGCA GCATGG (SEQ ID NO:
- 8);
- and
- wherein the primer pair comprises oligonucleotide primer sequences:
- (SEQ ID NO:
- 1)
- forward primer GCYGCTGCTGAAGAWATACAG,
- and
- (SEQ ID NO:
- 3)
- reverse primer CATCCCTCTACCAGATTTTCTGAAA.
Dependencies are inferred from the claim text.