Tumor-specific recognition molecules

A method of providing a molecule for tumor recognition comprising:

• a. providing a recombinant recognition molecule which comprises a variable heavy chain and a variable light chain and which specifically binds to core 1 antigen, wherein
• the variable heavy chain comprises (i) a CDRH1 region that comprises the amino acid sequence of SEQ ID NO:
• 1, or a variant thereof having at least 70% homology with SEQ ID NO:1;
• (ii) a CDRH2 region that comprises the amino acid sequence of any one of SEQ ID NO:
• 2-3, or a variant thereof having at least 70% homology with SEQ ID NO:2 or SEQ ID NO:3;
• and (iii) a CDRH3 region comprises the amino acid sequence of any one of SEQ ID NO:
• 4-6, or a variant thereof having at least 70% homology to any one of SEQ ID NO:4-6;
• and
• the variable light chain comprises (i) a CDRL1 region that comprises the amino acid sequence of any one of SEQ ID NO:
• 7-9, or a variant thereof having at least 70% homology to any one of SEQ ID NOS:7-9;
• (ii) a CDRL2 region that comprises the amino acid sequence of any one of SEQ ID NO:
• 10-11, or a variant thereof having at least 70% homology to any one of SEQ ID NO:10-11;
• and a CDRL3 region that comprises the amino acid sequence of any one of SEQ ID NO:
• 12-13 or a variant thereof having at least 70% homology to any one of SEQ ID NO:
• 12-13,
• b. modifying the recombinant recognition molecule, and
• c. selecting a modified recombinant recognition molecule whose core 1 antigen specificity is retained.