Fluorescent proteins and related methods and compounds
AssigneeUniversity of Massachusetts
InventorsEicke Latz, Brian G. Monks, Douglas T. Golenbock
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Abstract
The invention includes fusion polypeptides including a first fluorescent protein, e.g., a FRET donor protein, a second fluorescent protein, e.g., a FRET acceptor protein, and, linked to at least one of the fluorescent (e.g., FRET donor or FRET acceptor) proteins, an Fc-region of an immunoglobulin. The polypeptide can be immobilized with respect to a surface via the Fc-region even in the absence of antibodies to either the FRET donor protein or FRET acceptor protein, and can be used as a calibration standard for fluorescence resonance energy transfer includes a polypeptide.
Claims13
A composition comprising:
- a plurality of beads having different fluorescence resonance energy transfer (FRET) efficiencies, wherein immobilized on each of the beads are fusion proteins comprising:
- first and second fluorescent proteins, wherein the first and second fluorescent proteins are a FRET pair;
- a linker of a known length between the first and second fluorescent proteins;
- and
- an Fc region linked to one of the fluorescent proteins,
- the plurality of beads comprising fusion proteins with linkers of varied lengths to produce the different FRET efficiencies,
- wherein each bead comprises a marker so that beads with different FRET efficiencies can be identified in a mixture.
Dependencies are inferred from the claim text.