Method of hydrolyzing soluble starch with an alpha-amylase variant
AssigneeNovozymes A/S
InventorsAllan Svendsen, Carsten Andersen, Thomas Thisted, Claus Von Der Osten
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Abstract
The present invention relates to variants (mutants) of parent Termamyl-like alpha-amylases, which variant has alpha-amylase activity and exhibits altered properties relative to the parent alpha-amylase.
Claims4
A method of hydrolyzing soluble starch comprising contacting soluble starch with an alpha-amylase variant having alpha-amylase activity, the alpha-amylase variant comprising:
- (i) an amino acid sequence having at least 95% sequence identity to the amino acid sequence of SEQ ID NO:6, and
- (ii) a substitution that introduces a proline residue at a position corresponding to position 188 of the amino acid sequence of SEQ ID NO:6.
A method of hydrolyzing soluble starch comprising contacting soluble starch with an alpha-amylase variant having alpha-amylase activity, the alpha-amylase variant comprising:
- (i) an amino acid sequence having at least 96% sequence identity to the amino acid sequence of SEQ ID NO:6, and
- (ii) a substitution that introduces a proline residue at a position corresponding to position 188 of the amino acid sequence of SEQ ID NO:6.
A method of hydrolyzing soluble starch comprising contacting soluble starch with an alpha-amylase variant having alpha-amylase activity, the alpha-amylase variant comprising:
- (i) an amino acid sequence having at least 98% sequence identity to the amino acid sequence of SEQ ID NO:6, and
- (ii) a substitution that introduces a proline residue at a position corresponding to position 188 of the amino acid sequence of SEQ ID NO:6.
Dependencies are inferred from the claim text.