Porous particle reagent compositions, devices, and methods for biosensors

A biosensor sensor strip, for determining the concentration of an analyte in a sample, comprising:

• a sensor base at least partially covered by a lid;
• at least one reservoir formed by the sensor base, where the at least one reservoir encloses at least two conductors disposed on the base;
• and
• at least one reagent composition on at least one of the conductors to form a working electrode, the at least one reagent composition comprising,
• porous particles having an average diameter from 0.05 micrometer to 10 micrometer and a void volume of at least 20% (v/v);
• about 0.1 active unit to about 10 active units of at least one enzyme system per microliter of the at least one reagent composition,
• about 0.5% to about 10% (w/w) of at least one mediator, and
• at least one polymeric material;
• where the reservoir and the at least one reagent composition provide a maximum kinetic performance of a redox reaction of an analyte with the at least one reagent composition within less than about three seconds of introducing a sample to the sensor strip;
• and
• where the maximum kinetic performance is determined with a gated amperometric pulse sequence having at least five duty cycles, where
• each excitation of the duty cycles is 0.4 second in duration,
• each relaxation of the duty cycles is one second in duration,
• the relaxations are provided by an open circuit,
• at least three output current values are measured during each excitation,
• the excitations have a substantially constant potential of 250 mV, and
• the sample temperature is 23° C.

A method of determining the concentration of a biological analyte, comprising:

• introducing an aqueous sample including at least one biological analyte to a an electrode, the electrode comprising:
• at least one conductor;
• and
• at least one reagent composition disposed on the conductor, the reagent composition comprising:
• porous particles having an average diameter from 0.05 micrometer to 10 micrometer and a void volume of at least 20% (v/v);
• from about 0.5% to about 10% (w/w) of at least one mediator;
• at least one polymeric material;
• and
• at least one enzyme system;
• rehydrating the reagent composition with the aqueous sample;
• applying an input signal to the aqueous sample;
• measuring at least one output signal current value within about 0.4 to about 5 seconds of introducing the aqueous sample to the reagent composition;
• and
• determining the concentration of the at least one analyte in the aqueous sample from the at least one output signal current value.