Method of detecting leukemic cell
AssigneeNational University Corporation Hokkaido University
InventorsTakao Koike, Mitsufumi Nishio, Hiroshi Nishihara
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Abstract
[Problems] To provide a marker which is useful in diagnosing leukemia and a method of using the same. [Means for Solving Problems] A method of detecting a leukemia cell which comprises detecting a CD34-positive cell being negative in DOCK180 expression from a blood specimen. According to this method, a DOCK180 expression pattern specific to a tumor cell in leukemia, in particular, acute leukemia is confirmed so that information which is useful in diagnosing leukemia and monitoring and determining the severity and degree of recovery of the same by a doctor can be obtained by a convenient procedure using blood specimens and thus provided.
Claims10
A method of detecting a leukemia cell in a blood specimen, comprising the steps of:
- a) obtaining the blood specimen from an animal,
- b) analyzing the blood specimen to detect a CD34-positive cell,
- c) isolating the CD34-positive cell from the blood specimen, and
- d) analyzing the CD34-positive cell to detect if it is DOCK180-negative, wherein a CD34-positive-DOCK180-negative cell is a leukemia cell.
A method of detecting leukemia, comprising the steps of:
- a) obtaining a blood specimen from an animal,
- b) analyzing the blood specimen to detect a CD34-positive cell,
- c) isolating the CD34-positive cell from the blood specimen, and
- d) measuring an expression level of DOCK180 in the CD34-positive cell in the blood specimen to detect leukemia.
Dependencies are inferred from the claim text.