Methods for conferring immunity against protozoans in an animal
AssigneesKitasato Daiichi Sankyo Vaccine Co., Ltd., National Institute of Advanced Industrial Science and Technology
InventorsAkira Ito, Toru Gotanda, Shigeki Kobayashi, Katsumi Kume, Takeshi Matsumura
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Abstract
Immunity against protozoan is conferred on an animal by a method comprising orally administering to an animal a transformed plant cell comprising a polynucleotide encoding a protective antigen against protozoiasis development, a transformed plant or its progeny or clone comprising the transformed cell, a propagation material of the plant or its progeny or clone, a processed material or extract of the above cell, plant, or its progeny or clone, or propagation material, or a protective antigen against protozoiasis development isolated from the transformed plant cell or the transformed plant or its progeny or clone.
Claims14
A method for conferring immunity against the development of leucocytozoonosis in an animal comprising orally administering to said animal:
- (a) a transformed plant cell comprising a polynucleotide encoding a protective antigen against leucocytozoonosis development, wherein said protective antigen is expressed in said plant cell and wherein said polynucleotide comprises a nucleotide sequence encoding a protein comprising the amino acid sequence of SEQ ID NO:
- 1 or said polynucleotide comprises the nucleotide sequence of SEQ ID NO:
- 2;
- (b) a transformed plant comprising said transformed plant cell;
- (c) a progeny or clone of said transformed plant, wherein said progeny or clone comprises said polynucleotide;
- or
- (d) propagation material of said transformed plant or progeny or clone of said transformed plant;
- wherein said propagation material comprises the polynucleotide encoding said protective antigen;
- and
- wherein said administration of said plant cell, plant, progeny, clone, or propagation material comprising said protective antigen is in a dose sufficient to result in an increased serum antibody titer of antibodies against said protective antigen.
A method for conferring immunity against the development of leucocytozoonosis in an animal comprising orally administering to said animal:
- (a) a transformed plant cell comprising a polynucleotide encoding a protective antigen against leucocytozoonosis development, wherein said protective antigen is expressed in said plant cell and wherein said polynucleotide comprises a nucleotide sequence encoding a protein comprising the amino acid sequence of SEQ ID NO:
- 1;
- (b) a transformed plant comprising said transformed plant cell;
- (c) a progeny or clone of said transformed plant, wherein said progeny or clone comprises said polynucleotide;
- or
- (d) propagation material of said transformed plant or progeny or clone of said transformed plant, wherein said propagation material comprises the polynucleotide encoding said protective antigen;
- and
- wherein said administration of said plant cell, plant, progeny, clone, or propagation material comprising said protective antigen supplies said protective antigen in a dose sufficient to result in an increased serum antibody titer of antibodies against said protective antigen.
A method for conferring immunity against the development of leucocytozoonosis in an animal comprising orally administering to said animal:
- (a) a transformed plant cell comprising a polynucleotide encoding a protective antigen against leucocytozoonosis development, wherein said protective antigen is expressed in said plant cell and wherein said polynucleotide encoding a protective antigen against leucocytozoonosis development comprises the nucleotide sequence of SEQ ID NO:
- 2;
- (b) a transformed plant comprising said transformed plant cell;
- (c) a progeny or clone of said transformed plant, wherein said progeny or clone comprises said polynucleotide;
- or
- (d) propagation material of said transformed plant or progeny or clone of said transformed plant, wherein said propagation material comprises the polynucleotide encoding said protective antigen and
- wherein said administration of said plant cell, plant, progeny, clone, or propagation material comprising said protective antigen supplies said protective antigen in a dose sufficient to result in an increased serum antibody titer of antibodies against said protective antigen.
A method for conferring immunity against the development of leucocytozoonosis in an animal comprising orally administering to said animal a protective antigen against leucocytozoonosis development isolated from the transformed plant cell or the transformed plant or its progeny or clone, and administration of said protective antigen is in a dose sufficient to result in an increased serum antibody titer of antibodies against said protective antigen,
- wherein said plant cell, plant, progeny, clone, or propagation material comprising said protective antigen comprises a polynucleotide comprises a nucleotide sequence encoding a protein comprising the amino acid sequence of SEQ ID NO:
- 1 or said polynucleotide comprises the nucleotide sequence of SEQ ID NO:
- 2.
A method for conferring immunity against the development of leucocytozoonosis in an animal comprising orally administering to said animal:
- (a) a transformed plant cell comprising a polynucleotide encoding a protective antigen against leucocytozoonosis development, wherein said protective antigen is expressed in said plant cell and wherein said polynucleotide comprises a nucleotide sequence encoding a protein comprising the amino acid sequence of SEQ ID NO:
- 1 or said polynucleotide comprises the nucleotide sequence of SEQ ID NO:
- 2;
- (b) a transformed plant comprising said transformed plant cell;
- (c) a progeny or clone of said transformed plant, wherein progeny or clone comprises said polynucleotide;
- or
- (d) propagation material of said transformed plant or progeny or clone of said transformed plant;
- wherein said propagation material comprises the polynucleotide encoding said protective antigen;
- wherein said administration of said plant cell, plant, progeny, clone, or propagation material comprising said protective antigen is in a dose sufficient to result in an increased serum antibody titer of antibodies against said protective antigen and
- wherein said plant is an edible plant.
Dependencies are inferred from the claim text.